Intestinal Cell Differentiation and Phenotype in 2D and 3D Cell Culture Models.
Intestinal Cell Differentiation and Phenotype in 2D and 3D Cell Culture Models.
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2D 和 3D 细胞培养模型中的肠细胞分化和表型。
DOI：
10.1007/978-1-0716-3076-1_18
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Martínez-Espuga M
中科院分区：
文献类型：
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作者：
Martínez-Espuga M
三维（3D）培养模型比二维（2D）细胞培养模型更具有生理相关性。2D方法无法再现肿瘤微环境的复杂性，并且不太能够转化生物学见解;药物反应研究在临床上有许多局限性。在这里，我们使用Caco-2结肠癌细胞系，这是一种永生化的人类上皮细胞系，在特定条件下可以增殖和分化成绒毛样表型。我们描述了在二维和三维培养条件下的细胞分化和细胞生长，得出的结论是，细胞形态，极性，增殖和分化高度依赖于细胞培养系统的类型。
Three-dimensional (3D) culture models are more physiologically relevant than two-dimensional (2D) cell culture models. 2D approaches cannot reproduce the complexity of the tumor microenvironment and are less able to translate biological insights; and drug response studies have many limitations to be extrapolated to the clinics. Here, we use the Caco-2 colon cancer cell line, which is an immortalized human epithelial cell line that under specific conditions can polarize and differentiate into a villus-like phenotype. We describe cell differentiation and cell growth in both 2D and 3D culture conditions, concluding that cell morphology, polarity, proliferation and differentiation are highly dependent on the type of cell culture system.