Amino-acid change on the antigenic region B1 of H3 haemagglutinin may be a trigger for the emergence of drift strain of influenza A virus
Amino-acid change on the antigenic region B1 of H3 haemagglutinin may be a trigger for the emergence of drift strain of influenza A virus
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DOI：
10.1017/s0950268803001821
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发表时间：
2004-06-01
影响因子：
4.2
通讯作者：
Nakajima, K
中科院分区：
文献类型：
--
作者：
Sato, K;Morishita, T;Nakajima, K
用A/Alchi/2/68(A/AI/68)和A/悉尼/5/97(A/SD/97)株之间的嵌合血凝素(HA)蛋白，对1998年和1999年采集的对流感A/悉尼/5/97(H3N2)株具有血凝抑制(HI)活性的27份儿童和8名老年人的血清进行了鉴定。来自幼儿的血清倾向于只识别HA1区的抗原位点B1。另一方面，除D抗原点外，老年人的血清对HA1的所有抗原位点都有完全反应。最近流行的A/巴拿马/2007/99(A/PM/99)类病毒在抗原位点A、C和B2的氨基酸有差异，而B I没有。然而，即使是从幼儿获得的人抗血清对巴拿马样病毒也有HI活性。A/PM/99类病毒的有限流行可能是由于存在针对BI的抗体，该抗体是在A/SD/97类病毒感染时产生的。
Sera from 27 children and eight older persons, which had been collected in 1998 and 1999 and showed haemagglutination-inhibition (HI) activity against influenza A/Sydney/5/97 (H3N2) strain, were characterized with a binding assay using chimeric haemagglutinin (HA) proteins between A/Alchi/2/68 (A/AI/68) and A/Sydney/5/97 (A/SD/97) strains. Sera from the young children had a tendency to recognize only the antigenic site B1 of the HA1 region. On the other hand, sera of the older individuals were fully reactive to all antigenic sites of HA1 except antigenic site D. Recent epidemic strains, A/Panama/2007/99 (A/PM/99)-like viruses have differences in amino acids in antigenic sites A, C, and B2 but not B I. However, human antisera obtained even from young children had HI activity to Panama-like viruses. The limited epidemic of A/PM/99-like viruses may have been due to the existence of antibody against BI, which had been produced in response to infection by the A/SD/97-like viruses.