Transcriptional activating regions target a cyclin-dependent kinase
Transcriptional activating regions target a cyclin-dependent kinase
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DOI：
10.1073/pnas.232573899
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发表时间：
2002-11-12
影响因子：
11.1
通讯作者：
Ptashne, M
中科院分区：
文献类型：
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作者：
Ansari, AZ;Koh, SS;Ptashne, M
几个酵母激活剂被SRB10磷酸化，SRB10是一种与转录机制相关的细胞周期蛋白依赖的激酶。在每种情况下，磷酸化位点都在激活区域之外，并且这种修饰在不同的情况下具有不同的生理后果。我们在这里显示了某些酸性转录激活区与SRB10接触，正如在体内和体外所检测的那样。这种相互作用明显地定位了每个激活剂，因为它激活了转录，因此它被SRB10磷酸化，因此一种共同的机制将不同的底物靶向激酶。
Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidic transcriptional activating regions contact SRB10 as assayed both in vivo and in vitro. The interaction evidently positions each activator, as it activates transcription, so that it gets phosphorylated by SRB10, and thus a common mechanism targets disparate substrates to the kinase.