MONOCULM 3, an Ortholog of WUSCHEL in Rice, Is Required for Tiller Bud Formation
MONOCULM 3, an Ortholog of WUSCHEL in Rice, Is Required for Tiller Bud Formation
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MONOCULM 3 是水稻中 WUSCHEL 的直系同源物，是分蘖芽形成所必需的。
DOI：
10.1016/j.jgg.2014.12.005
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发表时间：
2015-02-20
影响因子：
5.9
通讯作者：
Li, Jiayang
中科院分区：
文献类型：
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作者：
Lu, Zefu;Shao, Gaoneng;Li, Jiayang
WUSCHEL (WUS) 在双子叶植物中对于维持分生组织活性起着至关重要的作用，但其在单子叶植物中的功能仍然难以捉摸。我们分离出一种新的单株突变体单株 3 (moc3)，其中点突变导致水稻 Oryza sativa WUS (OsWUS) 提前终止。形态学观察表明，moc3 中分蘖芽的形成受到破坏。 MOC3 位于细胞核中，可以与无顶相关蛋白 (TPR) 相互作用。 MOC3 的表达是由细胞分裂素诱导的，MOC3 的缺陷影响了几种二元细胞分裂素反应调节因子 OsRR 和 ORR 的表达。我们的结果表明 MOC3 是腋芽形成所必需的，并且与细胞分裂素具有复杂的关系。
WUSCHEL (WUS) plays an essential role for the maintenance of meristem activity in dicots, but its function is still elusive in monocots. We isolated a new monoculm mutant, monoculm 3 (moc3), in which a point mutation causes the premature termination of rice Oryza sativa WUS (OsWUS). Morphological observation revealed that the formation of tiller buds was disrupted in moc3. MOC3 was localized in the nuclear and could interact with TOPLESS-RELATED PROTEINS (TPRs). The expression of MOC3 was induced by cytokinins and defection of MOC3 affected the expression of several two-component cytokinin response regulators, OsRRs and ORRs. Our results suggest that MOC3 is required for the formation of axillary buds and has a complex relationship with cytokinins.