Production of a novel copolyester of 3-hydroxybutyric acid and medium chain length 3-hydroxyalkanaic acids by Pseudomonas sp 61-3 from sugars
Production of a novel copolyester of 3-hydroxybutyric acid and medium chain length 3-hydroxyalkanaic acids by Pseudomonas sp 61-3 from sugars
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DOI：
10.1007/s002530050697
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发表时间：
1996-04-01
影响因子：
5
通讯作者：
Doi, Y
中科院分区：
文献类型：
--
作者：
Kato, M;Bao, HJ;Doi, Y
假单胞菌61-3（分离自土壤）在以葡萄糖、果糖和甘露糖作为唯一碳源时产生由3-羟基丁酸（3 HB）和C-6、C-8、C-10和C-12的中链长3-羟基链烷酸（3 HA）组成的聚酯。生产的聚酯是均聚物和共聚物的共混物，其可以用沸腾的丙酮分馏。聚酯的丙酮不溶性部分是3-羟基丁酸酯单元的均聚物[聚（3 HB）]，而丙酮可溶性部分是含有范围从C-4至C-12的短链和中链长度的3-羟基链烷酸酯单元的共聚物[聚（3 HB-co-3 HA）]：44摩尔% 3-羟基丁酸酯、5摩尔% 3-羟基己酸酯、21摩尔% 3-羟基辛酸酯、25摩尔% 3-羟基癸酸酯、2摩尔% 3-羟基十二烷酸酯和3摩尔% 3-羟基-5-顺式-十二烯酸酯。通过C-13-NMR谱分析，证明该共聚物是3-羟基丁酸酯和中等链长的3-羟基链烷酸酯单元的无规共聚物。在不存在任何氮源的情况下，在细胞内由葡萄糖同时产生聚（3 HB）均聚物和聚（3 HB-co-3 HA）共聚物，这表明假单胞菌sp.61 -3具有两种类型的聚羟基烷酸酯合成，具有不同的底物特异性。
Pseudomonas sp. 61-3 (isolated from soil) produced a polyester consisting of 3-hydroxybutyric acid (3HB) and of medium-chain-length 3-hydroxyalkanoic acids (3HA) of C-6, C-8, C-10 and C-12, when sugars of glucose: fructose and mannose were fed as the sole carbon source. The polyester produced was a blend of homopolymer and copolymer, which could be fractionated with boiling acetone. The acetone-insoluble fraction of the polyester was a homopolymer of 3-hydroxybutyrate units [poly (3HB)], while the acetone-soluble fraction was a copolymer [poly(3HB-co-3HA)] containing both short- and medium-chain-length 3-hydroxyalkanoate units ranging from C-4 to C-12:44 mol% 3-hydroxybutyrate, 5 mol% 3-hydroxyhexanoate, 21 mol% 3-hydroxyoctanoate, 25 mol% 3-hydroxydecanoate, 2 mol% 3-hydroxydodecanoate and 3 mol% 3-hydroxy-5-cis-dodecenoate. The copolyester was shown to be a random copolymer of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoate units by analysis of the C-13-NMR spectrum. The poly(3HB) homopolymer and poly (3HB-co-3HA) copolymer were produced simultaneously within cells from glucose in the absence of any nitrogen source, which suggests that Pseudomonas sp. 61-3 has two types of polyhydroxyalkanoate syntheses with different substrate specificities.