SELECTIVE INVITRO GROWTH OF T-LYMPHOCYTES FROM NORMAL HUMAN BONE MARROWS
SELECTIVE INVITRO GROWTH OF T-LYMPHOCYTES FROM NORMAL HUMAN BONE MARROWS
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DOI：
10.1126/science.181845
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发表时间：
1976-01-01
期刊：
影响因子：
56.9
通讯作者：
GALLO, R
中科院分区：
文献类型：
--
作者：
MORGAN, DA;RUSCETTI, FW;GALLO, R
当将未分离的正常人骨髓细胞与从植物血凝素刺激的正常人淋巴细胞获得的条件培养基（Ly - CM）一起培养时，会发生T（胸腺衍生的）淋巴细胞的选择性生长。含高达90% T细胞的培养物维持了9个多月。这些T细胞表现出对Ly - CM的严格生长依赖性，并且爱泼斯坦 - 巴尔病毒信息始终呈阴性。
Selective growth of T [thymus-derived] lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90% T cells were maintained for more than 9 mo. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.