A self-powered glucose sensor based on BioCapacitor principle with micro-sized enzyme anode employing direct electron transfer type FADGDH
A self-powered glucose sensor based on BioCapacitor principle with micro-sized enzyme anode employing direct electron transfer type FADGDH
复制标题
基于生物电容器原理的自供电葡萄糖传感器，采用直接电子转移型 FADGDH 的微型酶阳极
DOI：
10.1088/2515-7655/abee32
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sode Koji
中科院分区：
文献类型：
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作者：
Lee Inyoung;Okuda-Shimazaki Junko;Tsugawa Wakako;Ikebukuro Kazunori;Sode Koji
糖尿病是一种身体不能产生足够的胰岛素或对胰岛素的正常反应的疾病;因此，血糖水平增加到异常高。因此，糖尿病的主要治疗是持续控制血糖水平。为了连续地控制血糖水平，已经开发了几种医疗设备来监测血糖水平，以用于血糖的自我监测的传感器和监测器为代表。从事医疗器械研发的人员的最终目标是开发可植入的生物设备，即自供电自主操作的人工胰腺系统。实现可植入人工胰腺的最具挑战性的问题之一是长期连续供电，目前依赖于可充电电池，需要定期更换。基于生物电容器原理，研制了一种直接电子转移型微型自供电酶葡萄糖传感器，其酶阳极面积为0.15mm × 0.75mm，电极面积仅为0.1mm2。结果，利用具有微尺寸酶阳极的生物燃料电池的生物电容器通过自供电操作。此外，基于生物电容器的充电/放电循环的频率，在13 mM至100 mM的范围内检测葡萄糖浓度。尽管为了监测低于13 mM的葡萄糖浓度范围，需要进一步提高微尺寸阳极的电流密度，但是这种具有基于生物电容器原理的微尺寸电极的自供电葡萄糖传感器在37 ℃下在100 mM磷酸钾缓冲液（pH7.0）中连续操作6.6小时。我们的成功表明，有可能实现自供电，自主和植入式传感模块的生物设备，如葡萄糖传感系统的人工胰腺。
Diabetes mellitus is a disorder in which the body does not produce enough or respond normally to insulin; consequently, blood glucose levels increase to become abnormally high. Accordingly, the primary treatment of diabetes is to control glycemic levels continuously. To continuously control glycemic levels, several medical devices have been developed to monitor blood glucose levels, represented by sensors and monitors for the self-monitoring of blood glucose. The ultimate goal for those engaged in research to develop medical devices is to develop implantable biodevices, namely self-powered autonomously operated artificial pancreas systems. One of the most challenging issues in realizing an implantable artificial pancreas is the long-term continuous supply of electricity, which is currently dependent on rechargeable batteries, requiring periodical replacement. In this work, we report the development of a direct electron transfer type enzyme-based miniaturized self-powered glucose sensor based on the BioCapacitor principle with a micro-sized enzyme anode area (0.15 mm× 0.75 mm), which has only 0.1 mm 2 of electrode surface. As a result, a BioCapacitor utilizing a biofuel cell with a micro-sized enzyme anode was operated by self-power. In addition, the glucose concentration was detected within the range from 13 mM to 100 mM based on the frequency of charge/discharge cycles of the BioCapacitor. Although further improvement of the current density of the micro-sized anode is necessary to monitor a glucose concentration range lower than 13 mM, this self-powered glucose sensor with a micro-sized electrode based on the BioCapacitor principle was operated continuously for 6.6 h at 37 C in 100 mM potassium phosphate buffer (pH 7.0). Our success indicates the potential to realize self-powered, autonomous, and implantable sensing modules for bio devices such as glucose-sensing systems for an artificial pancreas.