Proteomic analysis of human papillomavirus-related oral squamous cell carcinoma: Identification of thioredoxin and epidermal-fatty acid binding protein as upregulated protein markers in microdissected tumor tissue
Proteomic analysis of human papillomavirus-related oral squamous cell carcinoma: Identification of thioredoxin and epidermal-fatty acid binding protein as upregulated protein markers in microdissected tumor tissue
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DOI：
10.1002/pmic.200800882
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发表时间：
2009-04-01
期刊：
影响因子：
3.4
通讯作者：
von Eggeling, Ferdinand
中科院分区：
文献类型：
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作者：
Melle, Christian;Ernst, Guenther;von Eggeling, Ferdinand
人乳头瘤病毒（HPV）感染已被确定为口腔鳞状细胞癌（OSCC）的一个亚群的病因，发病率越来越高。HPV dna阳性可能会带来更好的预后，但相关的致癌机制尚不清楚。为了鉴定HPV相关蛋白，我们分析了HPV dna阳性（n = 17）和HPV dna阴性（n = 7）的OSCC组织样本的微解剖细胞。我们利用肽指纹图谱和SELDI MS鉴定了18种肿瘤组织蛋白，这些蛋白用2-DE分离。在蛋白质谱分析中检测到的许多信号中，我们发现硫氧还蛋白（TRX）和表皮脂肪酸结合蛋白在HPV相关肿瘤组织中上调。这项研究首次调查了微解剖HPV感染肿瘤组织的蛋白质组学变化，为病毒的致癌潜力提供了指示。
Human papillomavirus (HPV) infection has been identified as an etiologic agent for a subset of oral squamous cell carcinoma (OSCC) with increasing incidence. HPV DNA-positivity may confer better prognosis but the related oncogenic mechanisms are unknown. For the identification of HPV relevant proteins, we analyzed microdissected cells from HPV DNA-positive (n = 17) and HPV DNA-negative (n = 7) OSCC tissue samples. We identified 18 proteins from tumor tissues by peptide fingerprint mapping and SELDI MS that were separated using 2-DE. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified thioredoxin (TRX) and epidermal-fatty acid binding protein as upregulated in HPV related tumor tissue. This study, investigating for the first time proteomic changes in microdissected HPV infected tumor tissue, provides an indication on the oncogenic potential of viruses.