Gut microbiome is affected by gut region but robust to host physiological changes in captive active-season ground squirrels.
Gut microbiome is affected by gut region but robust to host physiological changes in captive active-season ground squirrels.
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DOI：
10.1186/s42523-021-00117-0
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发表时间：
2021-08-13
影响因子：
4.7
通讯作者：
Duddleston KN
中科院分区：
文献类型：
--
作者：
Grond K;Kurtz CC;Hatton J;Sonsalla MM;Duddleston KN
十三线地松鼠（Ictidomys tridecemlineatus）是专性冬眠动物，每年仅活动4-5个月。在此期间，松鼠迅速获得脂肪用于冬眠。我们研究了肠道微生物组在两个肠道部分的粘膜和管腔中的活跃季节如何变化：盲肠和回肠。我们对16 S rRNA基因进行测序，以评估松鼠肠道微生物组的多样性和组成，并使用差异丰度和网络分析来确定肠道部分之间的关系。盲肠和回肠之间以及回肠内粘膜和管腔之间的微生物组成存在显著差异。盲肠粘膜和管腔样品没有不同的α多样性和组成，并聚集个别松鼠。肠粘膜和管腔样品的群落组成不同，这可能是由于管腔中食物相关细菌的短暂性。在活动季节期间，我们没有检测到微生物组多样性和整体组成的变化，这表明松鼠微生物组可能对生理变化相对稳健。总的来说，我们发现13线地松鼠微生物组是由活跃季节的微环境塑造的。我们的研究结果为新的研究途径提供了基线数据，例如研究这些生理上独特的肠道环境中微生物功能的潜在差异。在线版本包含补充材料，可通过10.1186/s42523-021-00117-0获得。
Thirteen-lined ground squirrels (Ictidomys tridecemlineatus) are obligate hibernators and are only active 4–5 months annually. During this period, squirrels rapidly acquire fat for use during hibernation. We investigated how the gut microbiome changed over the active season in the mucosa and lumen of two gut sections: the cecum and ileum. We sequenced the 16S rRNA gene to assess diversity and composition of the squirrel gut microbiome and used differential abundance and network analyses to identify relationships among gut sections. Microbial composition significantly differed between the cecum and ileum, and within the ileum between the mucosa and lumen. Cecum mucosa and lumen samples did not differ in alpha diversity and composition, and clustered by individual squirrel. Ileum mucosa and lumen samples differed in community composition, which can likely be attributed to the transient nature of food-associated bacteria in the lumen. We did not detect a shift in microbiome diversity and overall composition over the duration of the active season, indicating that the squirrel microbiome may be relatively robust to changes in physiology. Overall, we found that the 13-lined ground squirrel microbiome is shaped by microenvironment during the active season. Our results provide baseline data for new avenues of research, such as investigating potential differences in microbial function among these physiologically unique gut environments. The online version contains supplementary material available at 10.1186/s42523-021-00117-0.