Fatty acid binding protein 4 promotes epithelial-mesenchymal transition in cervical squamous cell carcinoma through AKT/GSK3β/Snail signaling pathway
Fatty acid binding protein 4 promotes epithelial-mesenchymal transition in cervical squamous cell carcinoma through AKT/GSK3β/Snail signaling pathway
复制标题
脂肪酸结合蛋白4通过AKT/GSK3β/Snail信号通路促进宫颈鳞状细胞癌上皮间质转化
DOI：
10.1016/j.mce.2017.09.005
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发表时间：
2018-02-05
影响因子：
4.1
通讯作者：
Xie, Caifeng
中科院分区：
文献类型：
--
作者：
Jin, Jiangbo;Zhang, Ziyu;Xie, Caifeng
脂肪酸结合蛋白4 （FABP4）是脂肪酸结合蛋白家族的一员，参与多种生物细胞过程，包括肿瘤发生。然而，这种关键的脂肪因子在宫颈癌中的作用仍不清楚。在这项研究中，我们探讨了FABP4在宫颈癌中的功能及其潜在的分子机制。FABP4在宫颈鳞状细胞癌（CSCC）患者的组织样本中特异性升高，而在宫颈腺癌患者中无特异性升高，并且FABP4的水平与E-cadherin和Vimentin的表达相关。在体外，外源性FABP4以剂量依赖性的方式促进CSCC细胞的迁移和侵袭，并在F-Actin染色和tgf - β诱导的EMT实验中重组肌动蛋白细胞骨架。重要的是，AKT/GSK3 β /Snail通路似乎参与了fabp4诱导的CSCC细胞EMT。综上所述，我们的研究表明，FABP4的升高通过激活AKT/GSK3 β /Snail通路促进了CSCC中的EMT。(C) 2017 Elsevier B.V.版权所有
Fatty acid binding protein 4 (FABP4) is a member of the fatty acid binding protein family which involved in a variety of biological cellular processes, including tumorigenesis. However, the role of this key adipokine in cervical cancer is still unclear. In this study, we explored the function of FABP4 in cervical cancer and the underlying molecular mechanisms. FABP4 was specifically elevated in tissue samples from patients with cervical squamous cell carcinoma (CSCC) but not with cervical adenocarcinoma, and the level of FABP4 was correlated with E-cadherin and Vimentin expression. In vitro, exogenous FABP4 promoted the migration and invasion of CSCC cells in a dose-dependent manner, and reorganized the actin cytoskeletons in F-Actin staining and TGF-beta induced EMT assays. Importantly, the AKT/GSK3 beta/Snail pathway appears to be involved in FABP4-induced EMT in CSCC cells. In conclusion, our research demonstrated elevated FABP4 promoted EMT via the activation of AKT/GSK3 beta/Snail pathway in CSCC. (C) 2017 Elsevier B.V. All rights reserved.