A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics.
A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics.
复制标题
天然抗菌剂：基于串联质量标签蛋白质组学分析复方酚酸对大肠杆菌的抗菌效果及机制
DOI：
10.3389/fmicb.2021.738896
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发表时间：
2021
影响因子：
5.2
通讯作者：
Si H
中科院分区：
文献类型：
--
作者：
Zhang G;Yang Y;Memon FU;Hao K;Xu B;Wang S;Wang Y;Wu E;Chen X;Xiong W;Si H
本研究旨在探讨酚酸类化合物作为天然抗菌剂对多重耐药大肠杆菌的抗菌机制。大肠杆菌）。为此目的，将五种酚酸彼此组合，总共获得31种组合。为了选择最有效和最有效的组合，检测所有获得的组合的最小抑菌浓度（MIC），发现化合物酚酸（CPA）19（浓度分别为0.833、0.208和1.677 mg/mL的原儿茶酸、氢化肉桂酸和绿原酸）对E.与其他组合相比。此外，基于串联质量标签（TMT）蛋白质组学，CPA 19处理显著下调与多药耐药大肠杆菌的抗性相关的蛋白（Tsr、Tar、CheA和CheW）、OmpF和FliC。杆菌同时证明了CPA 19提高了E.大肠杆菌对抗生素（头孢曲松钠、阿莫西林、磷霉素、磺胺间甲氧嘧啶、加替沙星、林可霉素、氟苯尼考、头孢噻肟钠和利福平）的耐受性，导致鞭毛脱落，破坏细胞壁和细胞膜的结构，并导致大分子从细胞中泄漏。这一证据阐述了CPA 19的潜在治疗功效，并为抗菌剂的发现做出了重大贡献。
The objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most potent and effective combination, all of the obtained combinations were examined for minimum inhibitory concentration (MIC) and it was found that the compound phenolic acid (CPA) 19 (protocatechuic acid, hydrocinnamic acid, and chlorogenic acid at concentrations of 0.833, 0.208, and 1.677 mg/mL, respectively) showed better efficacy against E. coli compared to other combinations. Furthermore, based on tandem mass tag (TMT) proteomics, the treatment of CPA 19 significantly downregulated the proteins associated with resistance (Tsr, Tar, CheA, and CheW), OmpF, and FliC of multidrug-resistant E. coli. At the same time, we proved that CPA 19 improves the sensitivity of E. coli to antibiotics (ceftriaxone sodium, amoxicillin, fosfomycin, sulfamonomethoxine, gatifloxacin, lincomycin, florfenicol, cefotaxime sodium, and rifampicin), causes the flagellum to fall off, breaks the structure of the cell wall and cell membrane, and leads to macromolecules leaks from the cell. This evidence elaborated the potential therapeutic efficacy of CPA 19 and provided a significant contribution to the discovery of antibacterial agents.