Office Chromatography: Miniaturized All-in-One Open-Source System for Planar Chromatography
Office Chromatography: Miniaturized All-in-One Open-Source System for Planar Chromatography
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DOI：
10.1021/acs.analchem.8b02866
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发表时间：
2018-11-06
影响因子：
7.4
通讯作者：
Morlock, Gertrud E.
中科院分区：
文献类型：
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作者：
Fichou, Dimitri;Morlock, Gertrud E.
目前的高性能薄层色谱仪器是离线和逐步自动化的。然而，适度的小型化提供了许多优点，并且随着现代印刷和媒体技术向色谱（办公室色谱）领域的转移，它开辟了新的途径。这是证明在一个一体化的开源系统开发的平面色谱，特别是超薄层色谱。利用InkShield板控制热喷墨墨盒，在吸附剂层上以96 dpi的分辨率打印了皮升液滴。使用Marlin， 3D打印中流行的固件，打印头的笛卡尔运动可以完全控制打印过程。开发了开源软件来控制设备的每个操作步骤。样品溶液和流动相是喷墨打印的，例如用于分析染料或对羟基苯甲酸酯混合物溶液。对发光二极管（led）进行了研究。例如，深紫外led可以使用254纳米的光，而RGB led可以使用可见光范围。视频密度法得到相关系数大于0.999的校正函数。开发的模块化开源硬件紧凑（26 X 31 X 26 cm(3)），轻巧(
Current high-performance-thin-layer-chromatography instrumentation is offline and stepwise automated. However, moderate miniaturization offers many advantages and together with the transfer of modern print and media technologies to the field of chromatography (office chromatography) it opens up new avenues. This is demonstrated in an allin-one open-source system developed for planar chromatography and especially for ultrathin-layer chromatography. Using an InkShield board to control a thermal inkjet cartridge, picoliter drops were printed at a resolution of 96 dpi on the adsorbent layer. Using Marlin, a popular firmware in 3D printing, Cartesian movement of the print head was made possible for full control of the printing process. Open-source software was developed to control the device in each operation step. Sample solutions and mobile phase were inkjet-printed, exemplarily shown for the analysis of dye- or paraben-mixture solutions. Light-emitting diodes (LEDs) were investigated for documentation. For example, deep UV LEDs gave access to 254 nm light, and RGB LEDs gave access to the visible-light range. Calibration functions with correlation coefficients superior to 0.999 were obtained by videodensitometry. The developed modular open-source hardware was compact (26 X 31 X 26 cm(3)), light (