Complete Inhibition of the Rotation in a Barrierless TICT Probe for Fluorescence-On Qualitative Analysis
Complete Inhibition of the Rotation in a Barrierless TICT Probe for Fluorescence-On Qualitative Analysis
复制标题
完全抑制用于荧光定性分析的无障碍 TICT 探针的旋转
DOI：
10.1021/acs.analchem.2c02407
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发表时间：
2022
影响因子：
7.4
通讯作者：
Xincun Dou
中科院分区：
文献类型：
--
作者：
Guangfa Wang;Zhixin Wan;Zhenzhen Cai;Jiguang Li;Yushu Li;Xiaoyun Hu;Da Lei;Xincun Dou
抑制扭曲分子内电荷转移（TICT）是荧光分析中最有吸引力的方法之一，然而基于TICT的探针中的荧光是否可以被彻底点亮仍然是个谜。在这里，为了最大限度地提高基于TICT的探针的荧光信号，我们开发了一种模型，通过采用化学反应直接切割旋转电子供体和受体之间的连接，具有接近于零的预先设置的荧光信号。为了验证这一假设，通过将丙烯酰基与-C → C-识别位点接枝到香豆素上，成功地实现了具有无势垒旋转的非荧光探针，和7-当KMnO 4浓度低至0.95 nM和6.6 pg时，可在3 s内释放出具有明亮蓝色荧光的羟基香豆素。我们相信，本策略不仅可以加深对光化学的理解，而且还可以促进开发治疗诊断药物输送系统、能量转换、污染控制和降低健康风险。
Inhibition of twisting intramolecular charge transfer (TICT) is one of the most attractive methods for fluorescence-on analysis, whereas it remains enigmatic whether the fluorescence in a TICT-based probe could be thoroughly lightened. Here, for maximizing the fluorescence-on signal of the TICT-based probe, we develop a model by employing chemical reaction to directly cleave the linkage between the rotational electron donor and acceptor with a predisposed fluorescent signal close to zero. To validate this assumption, a nonfluorescent probe with barrierless rotation is successfully achieved by grafting acryloyl with -C═C- recognition sites onto coumarin, and 7-hydroxycoumarin with bright blue fluorescence could be released within 3 s upon probing KMnO4 with an amount as low as 0.95 nM and 6.6 pg. We believe that the present strategy could not only deepen the insights of photochemistry but also facilitate the development of a theranostic drug delivery system, energy conversion, pollution control, and health risk reduction.