Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells
Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells
复制标题
DOI：
10.1016/j.bbrc.2006.02.028
复制
发表时间：
2006-04-21
影响因子：
3.1
通讯作者：
Ishizaka, S
中科院分区：
文献类型：
--
作者：
Ouji, Y;Yoshikawa, M;Ishizaka, S
Wnt-10 b最初是从淋巴组织中分离出来的，并且已知其参与广泛的生物学作用。而最近发现其在毛囊发育的早期表达。然而，关于Wnt-10 b在皮肤上皮细胞分化中的作用的研究很少。为了评估其在上皮分化中的作用，我们使用亲和柱从伴刀豆球蛋白A刺激的淋巴细胞培养物的上清液中纯化Wnt-10 b，并研究其对成年小鼠来源的原代皮肤上皮细胞（MPSEC）分化的影响。用Wnt-10 b培养的MPSEC表现出从立方形到纺锤形的形态学变化，增殖受到抑制，并且还获得了毛干和毛囊内根鞘的特征，以红色Ayoub Shklar染色表示。以及免疫细胞学观察到的对AE-13和AE-15的反应。此外，RT-PCR分析表明，在Wnt-10 b处理的MPSEC中，角蛋白1、角蛋白2、兜甲蛋白、mHa 5和mHb 5的mRNA表达与基底细胞标志物角蛋白5的表达降低相关。此外，通过TCF报告基因（pTOPFLASH）测定证实了经典Writ信号通路的参与。提示Wnt-10 b促进MPSEC的分化，可能通过促进上皮细胞的分化在毛囊发育中发挥重要作用。(c)2006年爱思唯尔公司All rights reserved.
Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions. while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and investigated its effects oil the differentiation of adult mouse-derived primary skin epithelial cells (MPSEC). MPSEC cultured with Wnt-10b showed morphological changes from cuboidal to spindle-shaped with inhibited proliferation, and also obtained characteristics of the hair shaft and inner root sheath of the hair follicle, represented by red-colored Ayoub Shklar staining. and reactions to AE-13 and AE-15 as seen with immunocytology. Further, RT-PCR analysis demonstrated the expression of mRNA for keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5, in Wnt-10b-treated MPSEC. In addition, involvement of the canonical Writ signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. These results Suggest that Wnt-10b promotes the differentiation of MPSEC and may play an important role in hair follicle development by promoting differentiation of epithelial cells. (c) 2006 Elsevier Inc. All rights reserved.