CLASS-II MHC MOLECULES ARE SPECIFIC RECEPTORS FOR STAPHYLOCOCCUS ENTEROTOXIN-A
CLASS-II MHC MOLECULES ARE SPECIFIC RECEPTORS FOR STAPHYLOCOCCUS ENTEROTOXIN-A
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DOI：
10.1126/science.2658055
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发表时间：
1989-05-19
期刊：
影响因子：
56.9
通讯作者：
RICH, RR
中科院分区：
文献类型：
--
作者：
MOLLICK, JA;COOK, RG;RICH, RR
响应于葡萄球菌肠毒素A（SEA）刺激的T细胞增殖需要表达II类主要组织相容性复合体（MHC）分子的辅助细胞。用编码α-半乳糖苷酶的基因转染的鼠成纤维细胞。和β使用HLA-DR、DQ或DP的亚基来显示纯化的人T细胞对SEA的增殖应答依赖于II类分子，但不受应答者单倍型的限制。荧光标记的SEA与II类转染子的结合和用SEA-琼脂糖沉淀II类异二聚体表明增殖反应是SEA与II类分子结合的结果。该结合对II类分子是特异性的，并且不依赖于II类同种异型或同种型。SEA结合II类分子的能力可能是这类抗原的一般特征，现在称为“超抗原”。".
T cell proliferation in response to stimulation with Staphylococcus enterotoxin A (SEA) requires accessory cells that express class II major histocompatibility complex (MHC) molecules. Murine fibroblasts transfected with genes encoding the .alpha. and .beta. subunits of HLA-DR, DQ, or DP were used to show that the proliferative response of purified human T cells to SEA is dependent on class II molecules but is not restricted by the haplotype of the responder. Binding of fluoresceinated SEA to class II transfectants and precipitation of class II heterodimers with SEA-Sepharose show that the proliferative response is a result of SEA binding to class II molecules. The binding is specific for class II molecules and is independent of class II allotype or isotype. The ability of SEA to bind class II molecules may be a general characteristic of this class of antigens, now called "superantigens.".