Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue.
Very-low-density lipoprotein subfraction composition and metabolism by adipose tissue.
复制标题
极低密度脂蛋白亚组分的组成和脂肪组织的代谢。
DOI：
10.1016/s0026-0495(97)90001-9
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Coppack,SW
中科院分区：
文献类型：
--
作者：
Fisher,RM;Miles,JM;Kottke,BA;Frayn,KN;Coppack,SW
Lipoprotein lipase (LPL) plays a pivotal role in very—low-density lipoprotein (VLDL) metabolism. Within the circulation, the VLDL population is heterogeneous with respect to both size and composition. Several studies have investigated the action of LPL in vitro on different VLDL subfractions, but little is known of the action of LPL in vivo. To investigate this, arterial and adipose tissue venous plasma samples were obtained from 16 normal male healthy volunteers (aged 24.4 ± 1.8 years; body mass index, 23.5 ± 0.7 kg · m−2) following an overnight fast. VLDL subfractions were isolated (VLDL1of Sf60 to 400 and VLDL2of Sf20 to 60) and characterized in terms of triacylglycerol (TAG) and apolipoprotein (apo) B, E, CI, CII, and CIII content. The apolipoprotein content of VLDL1differed from that of VLDL2: the VLDL2fraction contained significantly more apo B (0.018 ± 0.004 v 0.011 ± 0.003 μmol · L−1, P = .001) but the ratios of TAG:apo B and apo CI:B, CII:B, and CIII:B were significantly higher in VLDL1(48,200 ± 7,960 v 13,860 ± 2,420, 22.7 ± 5.5 v 12.5 ± 2.2, 45.0 ± 6.3 v 14.9 ± 2.0, and 0.434 ± 0.077 v 0.357 ± 0.054, respectively, molar ratios, all P < .05). The venous blood draining an adipose tissue depot contained less VLDL1-TAG than arterial blood (328 ± 68 v 381 ± 83 μmol · L−1, respectively, P < .01), whereas VLDL2-TAG exhibited an opposite tendency (199 ± 46 v 172 ± 31 μmol · L−1, NS). Concentrations of VLDL1-apo B, -apo CII, and -apo CIII were significantly less in adipose tissue venous blood compared with arterial blood (0.011 ± 0.004 v 0.013 ± 0.004, 0.38 ± 0.08 v 0.43 ± 0.10, and 1.33 ± 0.35 v 1.58 ± 0.38 μmol · L−1, respectively, all P < .05). These studies demonstrated novel differences in VLDL1and VLDL2in terms of composition and metabolism by human adipose tissue LPL in vivo.
