Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus
Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus
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DOI：
10.1016/j.lfs.2006.06.002
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发表时间：
2006-09-27
期刊：
影响因子：
6.1
通讯作者：
Arai, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hama, Kotaro;Aoki, Junken;Arai, Hiroyuki
胚泡和接受子宫之间的相互作用对于成功着床是必不可少的。这个过程是由两种卵巢激素，孕酮和雌激素的及时相互作用调节的。然而，这些激素的分子靶点在很大程度上是未知的。我们最近发现，一种小的生物活性溶血磷脂，溶血磷脂酸，通过其细胞受体LPA在着床的建立中起着关键作用（3）。在这里，我们证明LPA（3）的表达是积极和消极的调节类固醇激素在小鼠子宫。子宫中LPA（3）mRNA水平在假孕早期增加，在性交后3.5天左右达到峰值（3.5 d.p.c.），4.5d降至基础水平。LPA（3）mRNA表达在卵巢切除小鼠中保持在低水平，给予孕酮可上调LPA（3）mRNA表达。此外，同时给予雌激素抵消了孕激素的作用。这些结果表明，孕激素和雌激素协同调节LPA（3）的表达，从而有助于在早期妊娠子宫的容受性。(c)2006爱思唯尔公司All rights reserved.
Reciprocal interactions between blastocysts and receptive uteri are essential for successful implantation. This process is regulated by the timely interplay of two ovarian hormones, progesterone and estrogen. However, the molecular targets of these hormones are largely unknown. We showed recently that a small bioactive lysophospholipid, lysophosphatidic acid, plays a pivotal role in the establishment of implantation via its cellular receptor, LPA(3). Here we demonstrate that LPA(3) expression is positively and negatively regulated by steroid hormones in mouse uteri. The LPA(3) mRNA level in the uteri increased during early pseudopregnancy, peaking around 3.5 days post coitus (3.5 d.p.c.), then, decreased to the basal level on 4.5 d.p.c. LPA(3) expression remained at a low level in ovariectomized mice, and administration of progesterone to ovariectomized mice up-regulated LPA(3) mRNA expression. In addition, simultaneous administration of estrogen counteracted the effect of progesterone. These results show that progesterone and estrogen cooperatively regulate LPA(3) expression, thereby contributing to the receptivity of uteri during early pregnancy. (c) 2006 Elsevier Inc. All rights reserved.