Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid
Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid
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疏水性室温离子液体中心肌细胞的收缩
DOI：
10.1016/j.bbrc.2012.09.068
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
and K. Morishima
中科院分区：
文献类型：
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作者：
T. Hoshino;K. Fujita;A. Higashi;K. Sakiyama;H. Ohno;and K. Morishima
室温离子液体（RTIL）作为一种新型的非水溶剂，在室温下取代有机溶剂和水溶剂用于液相化学过程，引起了人们的广泛关注。RTIL以其不挥发性、极低蒸气压、导电性和不燃性的特性而闻名。RTIL的这些独特性质引起了人们对它们在真空环境下用于扫描电子显微镜的生物细胞和组织的应用以及在微全分析系统（micro-TAS）的微流体装置中的关注。可居住的RTIL可以提高非水微TAS对活细胞的能力。一些RTIL似乎有能力在生物应用中取代水。然而，这些RTIL仅用于生物相容性测试的补充添加剂，用于固定细胞作为水溶液的替代品，以及用于简单分子。没有一种RTIL直接浸泡活细胞培养物。因此，我们证明了RTIL的设计，活细胞培养和液体电解质刺激收缩心肌细胞使用RTIL。我们使用搏动寿命来评估RTIL对心肌细胞的作用，以比较RTIL用于生物学应用的适用性。在氨基酸阴离子RTIL [P8，8，8，8][Leu]和[P8，8，8，8][Ala]、磷酸衍生物[P8，8，8，8][MeO（H）PO 2]和[P8，8，8，8][C7 CO2]中证实了心肌细胞的频繁自发收缩。RTIL的阴离子类型影响其对收缩心肌细胞的应用特性。这一结果表明，疏水基团RTIL的生物相容性设计，以实现与活细胞的生物应用的可能性。
Room-temperature ionic liquids (RTILs) are drawing attention as a new class of nonaqueous solvents to replace organic and aqueous solvents for chemical processes in the liquid phase at room temperature. The RTILs are notable for their characteristics of nonvolatility, extremely low vapor pressure, electric conductivity, and incombustibility. These distinguished properties of RTILs have brought attention to them in applications with biological cells and tissue in vacuum environment for scanning electron microscopy, and in microfluidic devices for micro-total analysis system (micro-TAS). Habitable RTILs could increase capability of nonaqueous micro-TAS for living cells. Some RTILs seemed to have the capability to replace water in biological applications. However, these RTILs had been applied to just supplemental additives for biocompatible test, to fixed cells as a substitute for an aqueous solution, and to simple molecules. None of RTILs in which directly soaks a living cell culture. Therefore, we demonstrated the design of RTILs for a living cell culture and a liquid electrolyte to stimulate contracting cardiomyocytes using the RTILs. We assessed the effect of RTILs on the cardiomyocytes using the beating lifetime to compare the applicability of RTILs for biological applications. Frequent spontaneous contractions of cardiomyocytes were confirmed in amino acid anion RTILs [P8,8,8,8][Leu] and [P8,8,8,8][Ala], phosphoric acid derivatives [P8,8,8,8][MeO(H)PO2], and [P8,8,8,8][C7CO2]. The anion type of RTILs had influence on applicable characteristics for the contracting cardiomyocyte. This result suggested the possibility for biocompatible design of hydrophobic group RTILs to achieve biological applications with living cells.