Nonsense-mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan
Nonsense-mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan
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DOI：
10.1016/j.ijfoodmicro.2007.05.003
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发表时间：
2007-07-15
影响因子：
5.4
通讯作者：
Fujii, Tateo
中科院分区：
文献类型：
--
作者：
Handa-Miya, Satoko;Kimura, Bon;Fujii, Tateo
InIA是参与单核细胞增生李斯特菌进入哺乳动物非吞噬细胞的表面蛋白。PrfA是一种正调控因子，调节一组毒力基因的表达。最近的研究表明，一些L。单核细胞增多性菌株由于其序列中的无义突变而具有这些蛋白质的截短形式，并且这些截短有助于该病原体的毒力的显著降低。本研究对L.从即食海产品中分离的单核细胞增多症揭示，59个分离物中只有一个具有无义突变的MIA，并且所有分离物都具有非突变的prfA。这表明，这些菌株可能是完全毒性的基础上，这些蛋白质的大小。(C)2007 Elsevier B.V.保留所有权利。
InIA is a surface protein participating in the entry of Listeria monocytogenes into mammalian non-phagocytic cells. PrfA is a positive regulatory factor that regulates the expression of a set of virulence genes. Recent studies revealed that some L. monocytogenes strains have a truncated form of these proteins because of nonsense mutations in their sequences, and these truncations contribute to the significant reduction in virulence of this pathogen. In this study, sequence analyses of inIA and prfA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated MIA and all had non-mutated prfA. This indicated that these strains could be fully virulent based on the sizes of these proteins. (C) 2007 Elsevier B.V. All rights reserved.