Cholesterol Crystals in Hepatocyte Lipid Droplets Are Strongly Associated With Human Nonalcoholic Steatohepatitis
Cholesterol Crystals in Hepatocyte Lipid Droplets Are Strongly Associated With Human Nonalcoholic Steatohepatitis
复制标题
DOI：
10.1002/hep4.1348
复制
发表时间：
2019-06-01
影响因子：
5.1
通讯作者：
Savard, Christopher
中科院分区：
文献类型：
--
作者：
Ioannou, George N.;Landis, Charles S.;Savard, Christopher
目前还不清楚是什么驱动了纤维化非酒精性脂肪性肝炎（NASH）的发展。我们的目的是确定肝细胞脂滴（LD）内的胆固醇结晶是否将纤维化NASH患者与孤立性肝脂肪变性患者区分开来，并研究导致肝细胞LD中胆固醇蓄积的途径。将患有纤维化NASH的患者（n=16）与患有孤立性脂肪变性的患者（n=14）进行比较。几乎所有纤维化NASH患者肝细胞LD中均有游离胆固醇被菲律宾蛋白染色（16/16）和胆固醇结晶（15/16），大多数与LD膜相关，而孤立性脂肪变性患者中仅有3/14例胆固醇结晶和3/14例菲律宾蛋白染色微弱（P
It is unclear what drives the development of fibrosing nonalcoholic steatohepatitis (NASH). We aimed to determine whether cholesterol crystallization within hepatocyte lipid droplets (LDs) distinguishes patients with fibrosing NASH from patients with isolated hepatic steatosis and to study pathways leading to cholesterol accumulation in hepatocyte LDs. Patients with fibrosing NASH (n=16) were compared to patients with isolated steatosis (n=14). Almost all patients with fibrosing NASH had free cholesterol staining by filipin (16/16) and cholesterol crystals (15/16) in hepatocyte LDs, mostly in association with the LD membrane, compared to only 3/14 with cholesterol crystals and 3/14 with faint filipin staining in patients with isolated steatosis (P