Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells
Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells
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DOI：
10.1016/s0047-6374(03)00009-5
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发表时间：
2003-04-01
影响因子：
5.3
通讯作者：
Blomberg, BB
中科院分区：
文献类型：
--
作者：
Frasca, D;Nguyen, D;Blomberg, BB
在本论文中，我们研究了衰老对脾B淋巴细胞中E2 A编码的转录因子E47的表达和功能的影响，这些淋巴细胞是未活化的或经不同刺激（LPS、抗-CD 40、抗-IgM，单独或与IL-4组合）活化的。结果表明，未受刺激的脾B细胞显示出非常低的E47蛋白水平以及E47 DNA结合活性，并且在B细胞活化后，E47表达和DNA结合活性在年轻小鼠中被强烈诱导，并且在老年小鼠中在显著较小的程度上被强烈诱导。在刺激脾B细胞的E47蛋白表达的水平被发现显着高于在老年小鼠，表明DNA结合活性与蛋白表达。这些结果共同表明，转录调节因子E47的表达减少可以帮助解释衰老小鼠中B细胞功能的降低。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.