The Secret Life of Tethers: The Role of Tethering Factors in SNARE Complex Regulation.
The Secret Life of Tethers: The Role of Tethering Factors in SNARE Complex Regulation.
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DOI：
10.3389/fcell.2016.00042
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发表时间：
2016
影响因子：
5.5
通讯作者：
Munson M
中科院分区：
文献类型：
--
作者：
Dubuke ML;Munson M
真核细胞的贩运是一个严格监管的过程，以确保货物正确输送到正确的目的地细胞器或质膜。在这篇综述中，我们重点关注囊泡融合机制（SNARE 复合物）如何通过多亚基束缚复合物 (MTC) 与 SNARE 和 Sec1/Munc18 (SM) 蛋白的相互作用进行调节。尽管这些因子用于膜运输的不同阶段，例如高尔基体到质膜的运输与液泡融合，以及各种不同的真核细胞类型，但它们功能之间的许多共性正在被揭示。我们探索了各种蛋白质-蛋白质相互作用和功能重建研究的结果，以突出它们的共同特征和调节模式的差异。这些研究是其他系统中机械探索的起点。
Trafficking in eukaryotic cells is a tightly regulated process to ensure correct cargo delivery to the proper destination organelle or plasma membrane. In this review, we focus on how the vesicle fusion machinery, the SNARE complex, is regulated by the interplay of the multisubunit tethering complexes (MTC) with the SNAREs and Sec1/Munc18 (SM) proteins. Although these factors are used in different stages of membrane trafficking, e.g., Golgi to plasma membrane transport vs. vacuolar fusion, and in a variety of diverse eukaryotic cell types, many commonalities between their functions are being revealed. We explore the various protein-protein interactions and findings from functional reconstitution studies in order to highlight both their common features and the differences in their modes of regulation. These studies serve as a starting point for mechanistic explorations in other systems.