Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate
Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate
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DOI：
10.1016/j.biortech.2011.03.099
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发表时间：
2011-06-01
影响因子：
11.4
通讯作者：
Adhikari, Basudam
中科院分区：
文献类型：
--
作者：
Chowdhury, Sougata Roy;Basak, Ratan Kumar;Adhikari, Basudam
巨大芽孢杆菌RB-05生长在葡萄糖和“tossa-daisee”（Corchorus olitorius）-衍生的黄麻，和生产和胞外多糖（EPS）的组成进行了监测。72小时后，葡萄糖和黄麻的EPS产量分别为0.065 +/- 0.013和0.297 g +/- 0.054 g（-1）底物。在黄麻胞外多糖生产中存在纤维素酶活性的细菌。高效液相色谱（HPLC）、基质辅助激光解吸/电离飞行时间质谱（MALDI-ToF）和傅立叶变换红外光谱（FT-IR）分析表明，黄麻培养液（JC）中合成的EPS与葡萄糖无机盐培养基（GMSM）中合成的EPS不同。而岩藻糖仅为次要成分（4.9 wt.%）在GMSM的EPS中，它是主要组分（41.9wt.%）在JC中合成的EPS。本研究建立了黄麻作为一种有效的发酵底物的EPS生产的纤维素酶产生菌。(C)2011爱思唯尔有限公司保留所有权利。
Bacillus megaterium RB-05 was grown on glucose and on "tossa-daisee" (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065 +/- 0.013 and of 0.297 g +/- 0.054 g(-1) substrate after 72 h was obtained for glucose and jute, respectively. EPS production in the presence of jute paralleled bacterial cellulase activity. High performance liquid chromatography (HPLC), matrix assisted LASER desorption/ionization-time of flight (MALDI-ToF) mass spectroscopy, and fourier transform infrared (FT-IR) spectroscopy demonstrated that the EPS synthesized in jute culture (JC) differed from that synthesized in glucose mineral salts medium (GMSM). While fucose was only a minor constituent (4.9 wt.%) of EPS from GMSM, it a major component (41.9 wt.%) of EPS synthesized in JC. This study establishes jute as an effective fermentation substrate for EPS production by a cellulase-producing bacterium. (C) 2011 Elsevier Ltd. All rights reserved.