Porcine Deltacoronavirus Infection and Transmission in Poultry, United States
Porcine Deltacoronavirus Infection and Transmission in Poultry, United States
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DOI：
10.3201/eid2602.190346
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发表时间：
2020-02-01
影响因子：
11.8
通讯作者：
Kenney, Scott P.
中科院分区：
文献类型：
--
作者：
Boley, Patricia A.;Alhamo, Moyasar A.;Kenney, Scott P.
冠状病毒在不同的宿主物种中引起呼吸道和胃肠道疾病。Deltacoronaviruses（DCoV）已在中国的各种鸣禽物种和豹猫中鉴定出。2009年，在亚洲猪的粪便样本中检测到猪三角洲冠状病毒（PDCoV），但直到2014年才确定其病原学作用，当时它在美国引起了猪的重大腹泻疫情。研究表明，PDCoV使用氨肽酶N蛋白的保守区域感染来自多个物种的细胞系，包括人、猪和鸡。由于PDCoV是一种潜在的人畜共患病病原体，因此对其在人类中的流行及其对人类疾病的贡献的研究仍在继续。我们报告了实验性PDCoV感染和随后在家禽中的传播。在PDCoV接种的雏鸡和火鸡幼雏中，我们观察到腹泻、泄殖腔和气管样品中持续的病毒RNA滴度、PDCoV特异性血清IgY抗体应答和肠道中的抗原阳性细胞。
Coronaviruses cause respiratory and gastrointestinal diseases in diverse host species. Deltacoronaviruses (DCoVs) have been identified in various songbird species and in leopard cats in China. In 2009, porcine deltacoronavirus (PDCoV) was detected in fecal samples from pigs in Asia, but its etiologic role was not identified until 2014, when it caused major diarrhea outbreaks in swine in the United States. Studies have shown that PDCoV uses a conserved region of the aminopeptidase N protein to infect cell lines derived from multiple species, including humans, pigs, and chickens. Because PDCoV is a potential zoonotic pathogen, investigations of its prevalence in humans and its contribution to human disease continue. We report experimental PDCoV infection and subsequent transmission among poultry. In PDCoV-inoculated chicks and turkey poults, we observed diarrhea, persistent viral RNA titers from cloaca) and tracheal samples, PDCoV-specific serum IgY antibody responses, and antigen-positive cells from intestines.