Denitrifying phosphorus removal from municipal wastewater and dynamics of "Candidatus Accumulibacter" and denitrifying bacteria based on genes of ppk1, narG, nirS and nirK.
Denitrifying phosphorus removal from municipal wastewater and dynamics of "Candidatus Accumulibacter" and denitrifying bacteria based on genes of ppk1, narG, nirS and nirK.
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DOI：
10.1016/j.biortech.2016.02.016
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发表时间：
2016-05
影响因子：
11.4
通讯作者：
W. Zeng;Jie Zhang;Anqi Wang;Yongzhen Peng
中科院分区：
文献类型：
--
作者：
W. Zeng;Jie Zhang;Anqi Wang;Yongzhen Peng
研究了“CandidatusAccumulibacter”的进化枝水平种群动态与城市废水反硝化除磷性能的相关性。连续流反应器缺氧区实现了稳定的反硝化除磷，占总除磷量的90%。 Accumulibacter谱系的IIC和IIF分支是反硝化除磷过程中的优势分支，达到ppk1克隆文库的90%。NarG基因文库表明Gamma和Beta变形菌在硝酸盐还原中发挥着重要作用。 nirS文库的多样性和丰度远高于nirK，成为执行亚硝酸盐还原的主要功能基因。根据nirS、nirK和ppk1的丰度计算，具有反硝化除磷能力的Accumulibacter占Accumulibacter总数的比例为22%。无论Accumulibacter是否带有narG基因，109个细胞/（g干污泥）的高丰度narG都能促进硝酸盐还原为亚硝酸盐，保证反硝化除磷性能。
Relevance of clade-level population dynamics of “CandidatusAccumulibacter” to performance of denitrifying phosphorus (P) removal from municipal wastewater was investigated. Stable denitrifying P removal in anoxic zone of continuous-flow reactor was achieved, accounting for 90% of total P removal. Clades IIC and IIF affiliated with Accumulibacter lineage were the dominant clades during denitrifying P removal, reaching 90% ofppk1 clone library.NarGgene library indicatedGammaandBeta-proteobacteriaplayed an important role in nitrate reduction. Diversity and abundance ofnirSlibrary was much more thannirK, and thus became the main functional gene to execute nitrite reduction. Based on abundance ofnirS,nirKandppk1, the ratio of Accumulibacter capable of denitrifying P removal to total Accumulibacter was 22%. No matter whether Accumulibacter hadnarGgene or not, high abundance ofnarGat a level of 109cells/(g dried-sludge) promoted nitrate reduced to nitrite, ensuring performance of denitrifying P removal.