Human coronavirus infections in rural Thailand: A comprehensive study using real-time reverse-transcription polymerase chain reaction assays
Human coronavirus infections in rural Thailand: A comprehensive study using real-time reverse-transcription polymerase chain reaction assays
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DOI：
10.1086/521308
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发表时间：
2007-11-01
影响因子：
6.4
通讯作者：
Erdman, Dean D.
中科院分区：
文献类型：
--
作者：
Dare, Ryan K.;Fry, Alicia M.;Erdman, Dean D.
背景我们试图确定感染人类冠状病毒（HCoV）229 E，OC 43，HKU 1和NL 63是否与肺炎相关，并确定泰国农村HCoV感染的流行病学。我们开发了一种实时逆转录聚合酶链反应（RT-PCR）检测面板识别HCoV类型，并比较2003年9月至2005年8月期间住院肺炎患者，门诊流感样疾病患者和无症状对照患者的HCoV感染。在研究第1年，734例肺炎患者中有43例（5.9%）感染HCoV; 72.1%的感染为OC 43。在研究第2年，当对照患者可用时，1156例肺炎患者中有21例（1.8%），513例门诊患者中有12例（2.3%），281例对照患者中有6例（2.1%）感染HCoV。与对照组相比，任何HCoV类型或所有类型合并感染与肺炎无关（所有HCoV类型的校正比值比为0.67 [ 95%置信区间为0.26 - 1.75]; P = 0.40）。HCoV感染在两个研究年中均被检测到;第一年93.6%的OC 43感染发生在1月至3月。HCoV感染很少在泰国农村地区使用敏感的实时RTPCR检测。我们没有发现HCoV感染和疾病之间的联系。然而，我们注意到HCoV毒株流行率的逐年变化，这可能影响了我们的结果。
Background. We sought to determine whether infections with human coronaviruses (HCoVs) 229E, OC43, HKU1, and NL63 are associated with pneumonia and to define the epidemiology of HCoV infection in rural Thailand.Methods. We developed a real-time reverse-transcription polymerase chain reaction (RT-PCR) assay panel for the recognized HCoV types and compared HCoV infections in patients hospitalized with pneumonia, outpatients with influenza-like illness, and asymptomatic control patients between September 2003 and August 2005.Results. During study year 1, 43 (5.9%) of 734 patients with pneumonia had HCoV infections; 72.1% of the infections were with OC43. During study year 2, when control patients were available, 21 (1.8%) of 1156 patients with pneumonia, 12 (2.3%) of 513 outpatients, and 6 (2.1%) of 281 control patients had HCoV infections. Compared with infection in control patients, infection with any HCoV type or with all types combined was not associated with pneumonia (adjusted odds ratio for all HCoV types, 0.67 [ 95% confidence interval, 0.26 -1.75]; P = .40). HCoV infections were detected throughout both study years; 93.6% of OC43 infections in the first year occurred from January through March.Conclusions. HCoV infections were infrequently detected in rural Thailand by use of sensitive real-time RTPCR assays. We found no association between HCoV infection and illness. However, we noted year-to-year variation in the prevalence of HCoV strains, which likely influenced our results.