Gene delivery by electroporation after dielectrophoretic positioning of cells in a non-uniform electric field
Gene delivery by electroporation after dielectrophoretic positioning of cells in a non-uniform electric field
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DOI：
10.1016/j.bioelechem.2008.01.006
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发表时间：
2008-04-01
影响因子：
5
通讯作者：
Wertheimer, Michael R.
中科院分区：
文献类型：
--
作者：
MacQueen, Luke A.;Buschmann, Michael D.;Wertheimer, Michael R.
我们报告了在电穿孔 (EP) 进行基因递送之前，使用介电电泳 (DEP) 将 U-937 单核细胞定位在非均匀电场内。 DEP 定位和 EP 脉冲都是使用一组在玻璃基板上微制造的通用惰性平面电极来完成的。电池介电特性的单壳模型和电场分布的有限元建模使我们能够预测电池定位的主要特征。发现电脉冲增加细胞膜对荧光分子和 pEGFPLuc DNA 质粒的渗透性的程度取决于先前的定位。对于给定的一组脉冲参数，EP 要么是不可逆的（导致细胞溶解），要么是可逆的（导致基因传递），或者不可检测，具体取决于细胞所处的位置。我们的结果清楚地表明，非均匀电场中细胞的位置依赖性 EP 可以通过 DER (C) 2008 Elsevier B.V. 保留所有权利。
We report the use of dielectrophoresis (DEP) to position U-937 monocytes within a non-uniform electric field, prior to electroporation (EP) for gene delivery. DEP positioning and EP pulsing were both accomplished using a common set of inert planar electrodes, micro-fabricated on a glass substrate. A single-shell model of the cell's dielectric properties and finite-element modeling of the electric field distribution permitted us to predict the major features of cell positioning. The extent to which electric pulses increased the permeability of the cell membranes to florescent molecules and to pEGFPLuc DNA plasmids were found to depend on prior positioning. For a given set of pulse parameters, EP was either irreversible (resulting in cytolysis), reversible (leading to gene delivery), or not detectable, depending on where cells were positioned. Our results clearly demonstrate that position-dependent EP of cells in a non-uniform electric field can be controlled by DER (C) 2008 Elsevier B.V. All rights reserved.