Affinity chromatography: A versatile technique for antibody purification
Affinity chromatography: A versatile technique for antibody purification
复制标题
DOI：
10.1016/j.ymeth.2016.12.010
复制
发表时间：
2017-03-01
期刊：
影响因子：
4.8
通讯作者：
Ayyar, B. Vijayalakshmi
中科院分区：
文献类型：
--
作者：
Arora, Sushrut;Saxena, Vikas;Ayyar, B. Vijayalakshmi
抗体在包括基础研究、成像、靶向递送、色谱法、诊断和治疗的无数应用中仍然是极其利用的实体。在生产阶段，抗体通常存在于复杂的基质中，并且它们的大多数预期应用需要纯化。由于需要高质量的产品和相关的高成本，抗体纯化一直是抗体下游加工的主要瓶颈。多年来，广泛的研究集中在寻找更好的纯化方法来克服这种滞留。在众多的抗体纯化技术中，亲和层析是最具选择性、快速、简便的抗体纯化方法之一。本文对亲和色谱及其纯化组分进行了综述。阵列的支持矩阵沿着与各种类别的亲和配体详细说明其基本的工作原理，连同每个系统的优点和局限性，在纯化不同类型的抗体，伴随着最近的发展和重要的实际方法的考虑，以优化纯化程序进行了讨论。(C)2016 Elsevier Inc. All rights reserved.
Antibodies continue to be extremely utilized entities in myriad applications including basic research, imaging, targeted delivery, chromatography, diagnostics, and therapeutics. At production stage, antibodies are generally present in complex matrices and most of their intended applications necessitate purification. Antibody purification has always been a major bottleneck in downstream processing of antibodies, due to the need of high quality products and associated high costs. Over the years, extensive research has focused on finding better purification methodologies to overcome this holdup. Among a plethora of different techniques, affinity chromatography is one of the most selective, rapid and easy method for antibody purification. This review aims to provide a detailed overview on affinity chromatography and the components involved in purification. An array of support matrices along with various classes of affinity ligands detailing their underlying working principles, together with the advantages and limitations of each system in purifying different types of antibodies, accompanying recent developments and important practical methodological considerations to optimize purification procedure are discussed. (C) 2016 Elsevier Inc. All rights reserved.