Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
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DOI：
10.1590/s0074-02762009000500018
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发表时间：
2009-08-01
期刊：
影响因子：
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通讯作者：
Zancopé-Oliveira, Rosely Maria
中科院分区：
文献类型：
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作者：
Reis, Rosani Santos;Almeida-Paes, Rodrigo;Zancopé-Oliveira, Rosely Maria
巴西里约热内卢正在流行孢子丝菌病，这是一种由真菌申克孢子丝菌引起的皮下真菌病，人类感染病例与接触猫有关。为了用分子方法证明这场疫情的人畜共患病特征，我们用基于DNA的分型方法对从疫情中分离的19株人和25株动物申克链球菌以及两个对照株进行了特征分析。采用3种不同的随机扩增多态性DNA(随机扩增多态性DNA)技术，结合来源于野生型噬菌体M13核心序列的小卫星DNA指纹分析技术对分离株进行分析。这些分析产生了具有相当多态的扩增产物。虽然分离株表现出很高的遗传亲缘关系，但它们可以聚为5-10个基因类型。申克链霉菌流行株与美国分离株的DNA图谱差异较大，两者的相似性为20%，而用M13扩增时相似性为60%。从猫指甲(42.8%)和口腔(66%)分离的申克链霉菌DNA指纹图谱与相关人类样本一致，表明此次疫情对动物和人类都有共同的感染源。很明显，猫是申克血吸虫传播的媒介。
An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25 animal S. schenckii isolates from the epidemic, as well as two control strains. To analyse the isolates, the random amplified polymorphic DNA (RAPD) technique was performed using three different primers, together with DNA fingerprinting using the minisatellite derived from the wild-type phage M13 core-sequence. The analyses generated amplicons with considerable polymorphism. Although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. The RAPD profiles of epidemic S. schenckii isolates could be distinguished from that of the United States isolate, displaying 20% similarity to each primer and 60% when amplified with the M13 primer. DNA fingerprinting of S. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. It is clear that cats act as a vehicle for dissemination of S. schenckii.