Probing Cd2+-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy
Probing Cd2+-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy
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DOI：
10.1021/acs.jpcc.6b00453
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发表时间：
2016-03-24
影响因子：
3.7
通讯作者：
Ding, Zhifeng
中科院分区：
文献类型：
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作者：
Li, Michelle S. M.;Filice, Fraser P.;Ding, Zhifeng
由于重金属对生物体的广泛危害，重金属细胞毒性已成为科学研究的支柱。尤其是Cd~(2+)可以激活广泛的生理通路，导致氧化应激、癌症形成或细胞死亡的显著增加。这在一定程度上是因为它能够在体内生物积累，排泄半衰期很长。在这里，我们使用亲水性氧化还原介体二茂铁羧酸盐、1‘1-二茂铁二羧酸盐和六胺基钚作为分子探针，在扫描电化学显微镜(SECM)中测定了镉对人膀胱癌(T24)单个活体细胞膜通透性的影响。亲水性和带电的感应剂应该对细胞是不渗透的。然而，当T24细胞被25mU的M-Cd~(2+)(t
Heavy metal cytotoxicity has become a mainstay in scientific research due to the vast range of detrimental effects on living organisms. Cd2+ in particular can activate a wide range of physiological pathways that can cause significant increases in oxidative stress, cancer formation, or cellular death. This is partly due to its ability to bioaccumulate in the body with a long half-life for excretion. Here, we used hydrophilic redox mediators, ferrocenecarboxylate, 1'1-ferrocene dicarboxylate and hexaamineruthenium, as molecular probes in scanning electrochemical microscopy (SECM) to determine the membrane permeability in single live human bladder cancer (T24) cells affected by Cd2+. Hydrophilic and charged sensing agents should be impermeable to the cells. However, when T24 cells were treated with 25 mu M Cd2+ (t