Revealing the carbohydrate pattern on a cell surface by super-resolution imaging
Revealing the carbohydrate pattern on a cell surface by super-resolution imaging
复制标题
通过超分辨率成像揭示细胞表面的碳水化合物模式
DOI：
10.1039/c4nr05970k
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发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Wang, Hongda
中科院分区：
文献类型：
--
作者：
Chen, Junling;Gao, Jing;Wang, Hongda
碳水化合物参与多种生理和病理活动，包括细胞粘附、信号传导和肿瘤侵袭。碳水化合物的分布是其多种功能的分子基础，但仍然知之甚少。在这里，我们采用直接随机光学重建显微镜（dSTORM），以可视化的模式N-乙酰葡糖胺（N-GlcNAc）的Vero细胞膜上的纳米级的分辨率。我们发现N-GlcNAc以不规则簇的形式存在于顶膜上，平均簇面积约为0.37 μ m（2）。大多数这些N-GlcNAc簇与脂筏共定位的双色dSTORM成像，表明碳水化合物与脂筏作为功能结构域密切相关。我们的研究结果表明，超分辨率成像能够在分子水平上表征碳水化合物在细胞表面的分布。
Carbohydrates are involved in various physiological and pathological activities including cell adhesion, signal transduction and tumor invasion. The distribution of carbohydrates is the molecular basis of their multiple functions, but remains poorly understood. Here, we employed direct stochastic optical reconstruction microscopy (dSTORM) to visualize the pattern of N-acetylglucosamine (N-GlcNAc) on Vero cell membranes at the nanometer level of resolution. We found that N-GlcNAcs exist in irregular clusters on the apical membrane with an average cluster area of about 0.37 mu m(2). Most of these N-GlcNAc clusters are co-localized with lipid rafts by dual-color dSTORM imaging, suggesting that carbohydrates are closely associated with lipid rafts as the functional domains. Our results demonstrate that super-resolution imaging is capable of characterizing the distribution of carbohydrates on the cellular surface at the molecular level.