In vivo quantification of subcutaneous and visceral adiposity by micro-computed tomography in a small animal model
In vivo quantification of subcutaneous and visceral adiposity by micro-computed tomography in a small animal model
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DOI：
10.1016/j.medengphy.2008.03.006
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Judex, S.
中科院分区：
文献类型：
--
作者：
Luu, Y. K.;Lublinsky, S.;Judex, S.
识别体内脂肪组织的数量和分布的准确和精确的技术对于脂肪发育、肥胖或糖尿病的研究至关重要。在这里，我们测试了体内微计算机断层扫描（microCT）是否可用于提供小鼠总脂肪、皮下脂肪和内脏脂肪体积分布的信息。对 90 只 C57BL/6J 小鼠（体重范围：15.7-46.5 g）在 5 个月龄时进行体内 microCT 扫描，随后处死。来自体内 microCT 的全身脂肪体积（颅底到远端胫骨）与离散性腺周（R-2 = 0.94）和皮下（R-2 = 0.91）脂肪垫的离体组织重量显着相关（p < 0.001）。将组织成分分析限制在 L1 和 L5 腰椎之间的腹部中段并没有改变总肥胖与移植脂肪垫重量之间的相关性。分割可以精确区分内脏脂肪和皮下脂肪，以及特定解剖区域内脂肪组织的量化。内脏脂肪垫重量和 microCT 之间的相关性决定了内脏脂肪体积 (R-2 = 0.95, p
Accurate and precise techniques that identify the quantity and distribution of adipose tissue in vivo are critical for investigations of adipose development, obesity, or diabetes. Here, we tested whether in vivo micro-computed tomography (microCT) can be used to provide information on the distribution of total, subcutaneous and visceral fat volume in the mouse. Ninety C57BL/6J mice (weight range: 15.7-46.5 g) were microCT scanned in vivo at 5 months of age and subsequently sacrificed. Whole body fat volume (base of skull to distal tibia) derived from in vivo microCT was significantly (p < 0.001) correlated with the ex vivo tissue weight of discrete perigonadal (R-2 = 0.94), and subcutaneous (R-2 = 0.91) fat pads. Restricting the analysis of tissue composition to the abdominal mid-section between L1 and L5 lumbar vertebrae did not alter the correlations between total adiposity and explanted fat pad weight. Segmentation allowed for the precise discrimination between visceral and subcutaneous fat as well as the quantification of adipose tissue within specific anatomical regions. Both the correlations between visceral fat pad weight and microCT determined visceral fat volume (R-2 = 0.95, p