The kinase CIPK14 functions as a negative regulator of plant immune responses to Pseudomonas syringae in Arabidopsis
The kinase CIPK14 functions as a negative regulator of plant immune responses to Pseudomonas syringae in Arabidopsis
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拟南芥中激酶 CIPK14 作为植物对丁香假单胞菌免疫反应的负调节因子
DOI：
10.1016/j.plantsci.2021.111017
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发表时间：
2021-08-21
期刊：
影响因子：
5.2
通讯作者：
Yang, Yi
中科院分区：
文献类型：
--
作者：
Ma, Yanlin;Chen, Qiaoqiao;Yang, Yi
作为植物中重要的第二信使，Ca2+ 参与许多生物过程，包括生物和非生物胁迫反应。 CBL 相互作用蛋白激酶（称为 CIPK）是 Ca2+ 介导的信号转导途径的重要组成部分。在这里，我们发现CIPK14在拟南芥的免疫反应调节过程中发挥作用。 CIPK14 功能缺失突变体对丁香假单胞菌表现出增强的抗性，而 CIPK14 过表达植物更容易受到细菌病原体的影响。 cipk14 突变体的抗性增强伴随着 SA 积累的增加和防御标记基因（PR1、EDS1、EDS5、ICS1）表达的升高。 CIPK14 的过表达抑制了 Pst DC3000、Pst DC3000 hrcC 和 flg22 诱导的 ROS 生成和胼胝质沉积。与野生型植物相比，flg22和elf18处理后，cipk14突变体中MPK3/6依赖性PTI标记基因（FRK1、CYP81F2、WAK2、FOX）的表达水平上调，但在CIPK14过表达植物中下调。此外，在 flg22 处理下，CIPK14 的功能丧失和功能获得均显着改变了 MPK3/6 的磷酸化状态，表明 CIPK14 在转录和转录后水平上都是植物免疫的通用调节剂。综上所述，我们的结果表明 CIPK14 在植物免疫反应中充当负调节因子。
As a critical second messenger in plants, Ca2+ is involved in numerous biological processes including biotic and abiotic stress responses. The CBL-interacting protein kinases, known as CIPKs, are essential components in Ca2+ - mediated signal transduction pathways. Here, we found that CIPK14 plays a role in the process of regulating immune response in Arabidopsis. The CIPK14 loss-of-function mutants exhibited enhanced resistance to the P. syringae, whereas CIPK14 overexpression plants were more susceptible to bacterial pathogen. Enhanced resistance in cipk14 mutants were accompanied by increased accumulation of SA and elevated expression of defense marker genes (PR1, EDS1, EDS5, ICS1). Overexpression of CIPK14 suppressed Pst DC3000, Pst DC3000 hrcC and flg22 induced generation of ROS and callose deposition. As compared with wild type plants, the expression levels of MPK3/6-dependent PTI marker genes (FRK1, CYP81F2, WAK2, FOX) were up-regulated in cipk14 mutants but down-regulated in CIPK14 overexpression plants after flg22 and elf18 treatment. Additionally, both loss-of-function and gain-of-function of CIPK14 significantly altered the phosphorylation status of MPK3/6 under flg22 treatment, suggesting that CIPK14 is a general modulator of plant immunity at both transcriptional and post-transcriptional level. Taken together, our results uncover that CIPK14 acts as a negative regulator in plant immune response.