Chlamydial MACPF protein CT153.
Chlamydial MACPF protein CT153.
复制标题
衣原体 MACPF 蛋白 CT153。
DOI：
10.1007/978-94-017-8881-6_13
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发表时间：
2014
影响因子：
--
通讯作者：
Nelson,DavidE
中科院分区：
文献类型：
--
作者：
Taylor,LaceyD;Nelson,DavidE
衣原体是专性细胞内细菌寄生虫，感染广泛的后生动物宿主。某些衣原体是人类眼部、生殖道和呼吸道慢性炎症性疾病的重要病因。位于衣原体基因组可变区的基因被称为可塑区，是致病多样性的关键决定因素。可塑区蛋白CT 153，只存在于选定的物种，包含一个膜攻击复合物/穿孔素（MACPF）域，它可以介导衣原体与宿主细胞的相互作用。CT 153存在于整个C.沙眼发育周期中，并加工成多肽，与膜的相互作用不同于亲本蛋白。衣原体从入侵的时间到它们最终离开宿主细胞与膜广泛地相互作用，因此可以想象MACPF蛋白在这些病原体的发病机制中的许多作用。在这里，我们提出了一个关于CT 153的概述，并强调了MACPF家族蛋白在一组病原体中的潜在作用，这些病原体的细胞内发育以与宿主细胞膜和细胞器的一系列相互作用为标志。最后，我们确定了新的战略，确定了可行的衣原体基因操作的基本工具集的最新发展CT 153功能。
Chlamydiae are obligate intracellular bacterial parasites that infect a wide range of metazoan hosts. SomeChlamydiaspecies are important causes of chronic inflammatory diseases of the ocular, genital and respiratory tracts in humans. Genes located in a variable region of chlamydial genomes termed the plasticity zone are known to be key determinants of pathogenic diversity. The plasticity zone protein CT153, present only in select species, contains a membrane attack complex/perforin (MACPF) domain, which may mediate chlamydial interactions with the host cell. CT153 is present throughout theC. trachomatisdevelopmental cycle and is processed into polypeptides that interact with membranes differently than does the parent protein. Chlamydiae interact extensively with membranes from the time of invasion until they eventually exit host cells, so numerous roles for a MACPF protein in pathogenesis of these pathogens are conceivable. Here, we present an overview of what is known about CT153 and highlight potential roles of a MACPF family protein in a group of pathogens whose intracellular development is marked by a series of interactions with host cell membranes and organelles. Finally, we identify new strategies for identifying CT153 functions made feasible by the recent development of a basic toolset for genetic manipulation of chlamydiae.