Immunopurification of Mitochondria from Arabidopsis.
Immunopurification of Mitochondria from Arabidopsis.
复制标题
拟南芥线粒体的免疫纯化。
DOI：
10.1002/cpz1.34
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yu Hong
中科院分区：
文献类型：
--
作者：
Luo Lilan;He Yajun;Li Jiayang;Yu Hong
由于叶绿体污染，从植物组织，特别是绿色叶中分离线粒体特别困难。与其他用于纯化植物线粒体的技术相比，免疫纯化方法具有许多优点：它在几分钟内快速纯化线粒体，避免线粒体代谢产物的损失，消除大多数其他细胞器（特别是叶绿体），保持分离的线粒体完整，需要很少的起始材料，并且具有纯化组织特异性线粒体的潜力。在这里，我们描述了一种快速的免疫纯化协议，线粒体分离，采用蛋白质特异性靶向外线粒体膜，作为免疫纯化处理。© 2021 Wiley Periodicals LLC.本文已于2022年7月19日更正。详见全文末尾。
Isolation of mitochondria from plant tissues, especially green leaves, is particularly difficult because of chloroplast contamination. Compared to other techniques for purifying plant mitochondria, the immunopurification method has a number of advantages: it rapidly purifies the mitochondria within several minutes, avoids loss of mitochondrial metabolites, eliminates most other organelles (especially the chloroplasts), keeps the isolated mitochondria intact, requires little starting material, and has the potential to purify tissue‐specific mitochondria. Here, we describe a rapid immunopurification protocol for mitochondrial isolation that employs a protein specifically targeted to the outer mitochondrial membrane that acts as an immunopurification handle. © 2021 Wiley Periodicals LLC.This article was corrected on 19 July 2022. See the end of the full text for details.