Behaviour of CD11b-Positive Cells in an Animal Model of Laser-Induced Choroidal Neovascularisation
Behaviour of CD11b-Positive Cells in an Animal Model of Laser-Induced Choroidal Neovascularisation
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DOI：
10.1159/000453550
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发表时间：
2017-01-01
期刊：
影响因子：
2.6
通讯作者：
Eter, Nicole
中科院分区：
文献类型：
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作者：
Li, Lu;Heiduschka, Peter;Eter, Nicole
背景/目的：免疫细胞，如视网膜的小胶质细胞，似乎参与了新生血管性老年性黄斑变性的病理过程。因此，本研究的目的是用免疫组织化学方法检测CD11b+免疫细胞在脉络膜新生血管形成(CNV)动物模型中各种因子和细胞因子的表达。方法：采用激光诱导小鼠CNV的动物模型。分别于激光治疗后第1、4、7、14天取眼。制备冰冻切片，用免疫组织化学方法检测小胶质细胞和CD11b免疫反应阳性的其他免疫细胞上是否存在不同的生长因子和细胞因子。结果：激光治疗4d后，激光斑点处CD11b+细胞数量显著增加，大部分CD11b+细胞可能是通过迁移进入激光斑点的。激光光斑中的CD11b+细胞表达多种促血管生成因子，如PDGF-β、成纤维细胞生长因子-1、成纤维细胞生长因子-2和转化生长因子-β(1)。一些炎性细胞因子也呈阳性，特别是肿瘤坏死因子-α、白介素6和CXCL1。在未经治疗的视网膜中，CD11b+细胞对这些蛋白几乎没有免疫反应。结论：小胶质细胞、巨噬细胞等CD11b+细胞可促进激光光斑新生血管形成，表现为中度炎症行为。在观察期间，发现这些分子中的大多数的免疫反应性降低。因此，免疫细胞活性的调节可能是一种减少CNV程度的工具。(C)2017年S.Karger AG，巴塞尔
Background/Aim: Immune cells, e.g. microglial cells of the retina, appear to be involved in pathological processes in neovascular age-related macular degeneration. Therefore, the purpose of this study was to immunohistochemically check the expression of various factors and cytokines by CD11b-positive (CD11b+) immune cells in an animal model of choroidal neovascularisation (CNV). Methods: We used the animal model of laser-induced CNV in mice. Eyes were isolated at 1, 4, 7, and 14 days after laser treatment. Cryosections were prepared and checked immunohistochemically for the presence of different growth factors and cytokines on microglial cells and other immune cells identified by CD11b immunoreactivity. Results: We found that the number of CD11b+ cells at the laser spots increased dramatically 4 days after laser treatment, the majority of them entering the laser spot most probably by migration. CD11b+ cells in the laser spot were positive for a variety of pro-angiogenic factors, such as PDGF-beta, FGF-1, FGF-2, and TGF-beta(1). They were also positive for some inflammatory cytokines, in particular TNF-alpha, IL-6, and CXCL1. In non-treated retinas, CD11b+ cells showed almost no immunoreactivity for these proteins. Conclusion: Microglial cells, macrophages, and other CD11b+ cells may promote the neovascularisation in the laser spot and show a moderate inflammatory behaviour. Immunoreactivity for most of these molecules was found to decrease during the time of observation. Modulation of immune cell activity may thus be a tool to reduce the extent of CNV. (C) 2017 S. Karger AG, Basel