First CRF01_AE/B recombinant of HIV-1 is found in Thailand.
First CRF01_AE/B recombinant of HIV-1 is found in Thailand.
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第一个 HIV-1 的 CRF01_AE/B 重组体在泰国发现。
DOI：
10.1097/00002030-200105250-00018
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
Jean K. Carr
中科院分区：
文献类型：
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作者：
S. Tovanabutra;V. Polonis;Mark de Souza;R. Trichavaroj;P. Chanbancherd;Bohye Kim;E. Sanders;S. Nitayaphan;Arthur E. Brown;Merlin R. Robb;D. Birx;F. McCutchan;Jean K. Carr
在泰国，HIV-1疫情于1988年突然开始，引入了B亚型和E亚型，现在称为循环重组形式(CRF)，CRF01_AE。这两种毒株在不同的高危人群中独立出现[1]：注射吸毒者(IDU)中的B亚型和异性接触者中的CRF01_AE[2，3]。HIV-1 B亚型在曼谷吸毒人员中仍然很常见，但1995-1998年调查的吸毒人员中有80%发现了CRF01_AE。1994年观察到HIV-1 B亚型和CRF01_AE双重感染[5]，这为泰国疫情中这两个亚型之间的重组提供了机会。虽然CRF01_AE和B亚型之间的重组已经在黑猩猩的实验性双重感染中发生[6]，但在人类中还没有描述过这样的重组。在这里，我们确定了在泰国一名多次暴露的人中发现的HIV-1的AE/B亚型间重组。1997年，对一名40岁泰国男子(NP1623)的病毒亚型进行了筛查，发现基因组不同部分的亚型不一致。V3环肽酶免疫分析[7]将NP1623的血清分类为CRF01_AE，包膜异源双链迁移率分析[8]证实了这一命名。然而，GAG前导区[9]的限制性片段长度多态性分析表明，NP1623感染了B亚型，gp41[10]的差异聚合酶链式反应(PCR)分析也是如此。这些结果表明gp120亚型和其他病毒亚型之间不一致。
In Thailand, the HIV-1 epidemic started abruptly in 1988 with the introduction of subtype B and subtype E, now called the circulating recombinant form (CRF), CRF01_AE. These two strains appeared independently in distinct high-risk populations [1]: subtype B among injecting drug users (IDU) and CRF01_AE among those who were heterosexually exposed [2, 3]. HIV-1 subtype B is still common among infected Bangkok IDU, but CRF01_AE was found in 80% of IDU surveyed in 1995–1998 [4]. Dual infection with HIV-1 subtype B and CRF01_AE was observed by 1994 [5], providing the opportunity for recombination between these two subtypes in the Thailand epidemic. Whereas recombination between CRF01_AE and subtype B has occurred in an experimental dual infection of a chimpanzee [6], such a recombinant in humans has not yet been described. Here, we identify an AE/B inter-subtype recombinant of HIV-1 found in a multiply exposed individual in Thailand. The full-length genome of this recombinant has been analysed and characterized.In 1997, screening assays for the subtype of the virus of a 40-year-old Thai man (NP1623) provided evidence of subtype discordance in different parts of the genome. A V3 loop peptide enzyme immunoassay [7] classified the serum of NP1623 as CRF01_AE and an envelope heteroduplex mobility assay [8] confirmed that designation. A restriction fragment length polymorphism analysis from the gag leader region [9], however, indicated that NP1623 was infected with subtype B, as did a differential polymerase chain reaction (PCR) assay in gp41 [10]. These results suggested discordance between the subtype of gp120 and the subtype of the rest of the virus.
DOI：
10.1101/gr.4.5.s202
发表时间：
1995-04
期刊：
PCR methods and applications
影响因子：
--
作者：
Eric Delwart;B. Herring;Allen G. Rodrigo;James I. Mullins
通讯作者：
Eric Delwart;B. Herring;Allen G. Rodrigo;James I. Mullins