Alternatively spliced sodium channel transcripts expressed in field strains of the diamondback moth.
Alternatively spliced sodium channel transcripts expressed in field strains of the diamondback moth.
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DOI：
10.1016/j.ibmb.2008.06.006
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发表时间：
2008-09
影响因子：
3.8
通讯作者：
S. Sonoda;Chikako Igaki;H. Tsumuki
中科院分区：
文献类型：
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作者：
S. Sonoda;Chikako Igaki;H. Tsumuki
L1014 F和T929 I突变的频率，这两个都是参与神经不敏感的抗性拟除虫菊酯，在现场和实验室菌株的小菜蛾，小菜蛾在DNA和RNA水平进行了检查。结果表明，在L1014 F和T929 I位点上，野毒株的抗性等位基因频率分别为82.8 ~ 100%和72.9- 94.4%。没有观察到L1014 F突变的转录后调控。根据相互排斥的外显子18 a和18 b的表达模式，将所检测的昆虫分为四组。大多数昆虫在现场菌株中表达的转录本含有外显子18 b比那些含有外显子18 a更丰富，虽然这两个转录本在实验室菌株的所有昆虫中以相似的比例表达。一些其它昆虫表达包含外显子18 a和18 b的部分的嵌合转录物。推导的嵌合转录本的氨基酸序列编码对应于家蝇中的超级kdr突变（M918 T）的位点处的从Met到Ile的氨基取代。M918 I突变频率为5.0- 19.4%。对基因组组织的分析表明，嵌合序列在基因组中编码。
The frequencies of the L1014F and T929I mutations, both of which are involved in nerve insensitive resistance to pyrethroids, were examined in field and laboratory strains of the diamondback moth, Plutella xylostella at DNA and RNA levels. Results showed that the resistance allele frequencies at the L1014F and T929I sites in the field strains were respectively, 82.8–100% and 72.9–94.4%. No posttranscriptional regulation of the L1014F mutation was observed. The examined insects were classifiable into four groups according to the expression patterns of mutually exclusive exons 18a and 18b. Most insects in the field strains expressed transcripts containing exon 18b more abundantly than those containing exon 18a, although both transcripts were expressed with similar proportions in all insects of the laboratory strains. Some other insects expressed a chimeric transcript comprising parts of exons 18a and 18b. Deduced amino acid sequences of the chimeric transcript encoded amino substitution from Met to Ile at the site corresponding to the super-kdr mutation (M918T) in Musca domestica. The frequencies of the M918I mutation in the field strains were 5.0–19.4%. Analyses of the genomic organization revealed that the chimeric sequences are encoded in the genome.