Marker-assisted Analysis for Soybean Hard Seededness with Isozyme and Simple Sequence Repeat Loci
Marker-assisted Analysis for Soybean Hard Seededness with Isozyme and Simple Sequence Repeat Loci
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DOI：
10.1270/jsbbs.54.133
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发表时间：
2004-06
期刊：
影响因子：
2.4
通讯作者：
S. Sakamoto;J. Abe;A. Kanazawa;Y. Shimamoto
中科院分区：
文献类型：
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作者：
S. Sakamoto;J. Abe;A. Kanazawa;Y. Shimamoto
测定了野生大豆（Glycine max亚种）的基因组区域。携带硬粒性数量性状位点（QTL），这是野生大豆和栽培大豆之间的一个明显特征。用种皮渗透性（SCP）评价了野生大豆与栽培大豆杂交后代的硬粒性分离。f2和f3的高亲代相关系数表明SCP是一个高度遗传的性状。对5个同工酶位点和131个SSR位点的标记辅助分析表明，至少有两个主要QTL和一个次要QTL参与了该性状的控制。分子连锁组D1b+W中SSR标记Satt459附近的QTL对SCP的影响最大，分别占f2和f3平均表型方差的23.8%和38.5%。由抑制种皮颜色基因座(I)和短柔毛颜色基因座(T)决定的种皮颜色在f4至f6中也与SCP密切相关，尽管在f2中种皮颜色似乎与SCP无关。本研究和前人的研究结果表明，不同QTL对野生和栽培大豆种皮通透性的表型差异有不同程度的影响。
We determined the genomic regions of wild soybean (Glycine max subsp. soja) carrying quantitative trait loci (QTL) for hard seededness, a distinct character between the wild and cultivated soybeans. The segregation of hard seededness, as evaluated using seed coat permeability (SCP), was examined in the progeny of a cross between wild and cultivated soybeans. The high parent-offspring correlation coefficient between F 2 and F 3 indicated that SCP was a highly heritable character. A marker-assisted analysis of five isozyme and 131 simple sequence repeat (SSR) loci revealed that at least two major and one minor QTL were involved in controlling the trait. The QTL near an SSR marker (Satt459) in molecular linkage group D1b+W had the greatest effect on SCP, and accounted for 23.8 and 38.5% of the phenotypic variance observed in the F 2 and F 3 mean, respectively. Seed coat color, determined by a locus inhibiting seed coat color (I) and a pubescence color locus (T), was also closely associated with SCP in F 4 to F 6 , although seed coat color did not appear to be associated with SCP in the F 2 . The results of this and a previous study suggest that different sets of QTL contribute to the phenotypic difference of seed coat permeability in wild and cultivated soybeans to different degrees.