Expression and prognostic significance of a new tumor metastasis suppressor gene LASS2 in human bladder carcinoma
Expression and prognostic significance of a new tumor metastasis suppressor gene LASS2 in human bladder carcinoma
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DOI：
10.1007/s12032-011-0026-6
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发表时间：
2012-09-01
期刊：
影响因子：
3.4
通讯作者：
Ke, Changxin
中科院分区：
文献类型：
--
作者：
Wang, Haifeng;Wang, Jiansong;Ke, Changxin
LASS2基因是一种新的肿瘤转移抑制基因，与前列腺癌、乳腺癌、肝癌、卵巢癌和胰腺癌的侵袭程度和复发程度有关。然而，LASS2在人类膀胱癌中的表达及其预后意义在很大程度上是未知的。本研究采用免疫组化染色法检测80例不同分期患者中LASS2蛋白的表达。LASS2在膀胱癌中的预后价值也可以通过长期随访调查来评估。采用实时定量PCR （real-time quantitative PCR, qPCR）技术检测LASS2基因在30例膀胱全切除术患者膀胱癌及配对非肿瘤膀胱组织中的mRNA表达水平。我们发现lass2阴性膀胱癌患者的临床预后较差。LASS2 mRNA的表达与临床分期有显著相关性(P
The LASS2 gene has been identified as a new tumor metastasis suppressor gene and has been seen to correlate with the degree of invasion and recurrence in carcinomas of prostate, breast, liver, ovarian, and pancreas. However, expression and prognostic significance of LASS2 in human bladder carcinoma are largely unknown. In this study, the protein expression of LASS2 in 80 patients with different stages was detected by immunohistochemical staining. The prognostic value of LASS2 in bladder cancers can also be assessed by a long-term follow-up investigation. The mRNA expression level of the LASS2 gene was examined using real-time quantitative PCR (qPCR) in human bladder carcinoma and paired non-tumor bladder tissues, which were obtained from 30 patients who underwent total cystectomy. We found that patients with LASS2-negative bladder cancer were linked to poor clinical prognosis. The expression of LASS2 mRNA was significantly correlated with clinical stage (P