In Situ Generation of Hydroxyapatite on Biopolymer Particles for Fabrication of Bone Scaffolds Owning Bioactivity
In Situ Generation of Hydroxyapatite on Biopolymer Particles for Fabrication of Bone Scaffolds Owning Bioactivity
复制标题
生物聚合物颗粒上原位生成羟基磷灰石用于制造具有生物活性的骨支架
DOI：
10.1021/acsami.0c13768
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发表时间：
2020-10-14
影响因子：
9.5
通讯作者：
Min, Anjie
中科院分区：
文献类型：
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作者：
Feng, Pei;Peng, Shuping;Min, Anjie
羟基磷灰石(HAP)可以赋予生物聚合物支架良好的生物活性和骨传导能力，但HAP与生物聚合物之间的界面结合较弱。本研究在聚L-乳酸(PLLA)粒子上原位生成羟基磷灰石，并将其用于选择性激光烧结支架材料。详细地说，PLLA粒子首先通过氧化多巴胺聚合来功能化，在粒子表面引入了大量的活性邻苯二酚基团，然后邻苯二酚基团通过螯合作用在模拟体液中浓缩了Ca~(2+)离子，然后，Ca~(2+)离子通过静电作用吸附PO43-离子，从而原位成核HAP。结果表明，HAP在PLLA颗粒表面生成均匀，HAP与PLLA在HAP/PLLA支架中有良好的界面结合。同时，该支架通过诱导磷灰石沉淀而显示出良好的生物活性，为人骨髓间充质干细胞的附着、增殖和成骨分化提供了良好的环境。更重要的是，支架在体内能够促进血管的生长和新骨的形成，骨体积分数和骨密度分别比纯PLLA支架提高了44.44%和41.73%。血清生化指标均在正常范围内，说明支架植入后对人体正常功能无不良影响。
Hydroxyapatite (HAP) can endow a biopolymer scaffold with good bioactivity and osteoconductive ability, while the interfacial bonding is fairly weak between HAP and biopolymers. In this study, HAP was in situ generated on poly(L-lactic acid) (PLLA) particles, and then they were used to fabricate a scaffold by selective laser sintering. Detailedly, PLLA particles were first functionalized by dopamine oxide polymerization, which introduced abundance active catechol groups on the particle surface, and subsequently, the catechol groups concentrated Ca2+ ions by chelation in a simulated body fluid solution, and then, Ca2+ ions absorbed PO43- ions through electrostatic interactions for in situ nucleation of HAP. The results indicated that HAP was homogeneously generated on the PLLA particle surface, and HAP and PLLA exhibited good interfacial bonding in the HAP/PLLA scaffolds. Meanwhile, the scaffolds displayed excellent bioactivity by inducing apatite precipitation and provided a good environment for human bone mesenchymal stem cell attachment, proliferation, and osteogenic differentiation. More importantly, the ingrowth of blood vessel and the formation of new bone could be stimulated by the scaffolds in vivo, and the bone volume fraction and bone mineral density increased by 44.44 and 41.73% compared with the pure PLLA scaffolds, respectively. Serum biochemical indexes fell within the normal range, which indicated that there was no harmful effect on the normal functioning of the body after implanting the scaffold.