Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft
Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft
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DOI：
10.1016/j.biomaterials.2006.11.012
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Lin, Feng-Huei
中科院分区：
文献类型：
--
作者：
Wu, Hsi-Chin;Wang, Tzu-Wei;Lin, Feng-Huei
在这项研究中，我们评估了一种直径2 mm、长1 cm的可生物降解的胶原蛋白小径血管移植物的可行性。综上所述，采用真空抽吸和冷冻干燥的方法制备了双层I型胶原膜。将平滑肌细胞接种到多孔膜的下侧，将内皮细胞接种到多孔膜的上光滑侧。培养7d后，收获血管替代物进行体外检测或体内植入皮下层进行生物相容性测试，然后将管状血管假体作为临时可吸收导向器，作为体内血管移植物，促进大鼠下腔静脉完全再生。植入12周后，血管腔内可见一层连续的内皮细胞和一层较薄的平滑肌细胞，血管腔内可见一层连续的内皮细胞和一层光滑的中膜。组织学结果显示，没有明显的血栓形成和内膜增生的迹象。该组织工程化血管替代物不仅具有足够的抗张强度和良好的生物相容性，而且具有促进血管再生的作用。在未来，我们认为这种可生物降解的血管替代物将为人工血管中的小直径人工血管提供应用的可能性。(C)2006爱思唯尔有限公司。保留所有权利。
In this study, we have evaluated the feasibility of developing a biodegradable collagenous small diameter vascular graft of 2mm diameter and 1 cm length. In brief, bi-layer type I collagen membrane was fabricated under vacuum suction and lyophilization methods. The smooth muscle cells were inoculated into the lower side of the porous membrane, while endothelial cells were seeded onto upper smooth side of the membrane. After cultured for 7 days, the vascular substitute was either harvested for in vitro examination or in vivo implanted in the subcutaneous layer for biocompatibility test.The tubular vascular prosthesis was then used as a temporary absorbable guide that served as an in vivo vascular graft to promote the complete regeneration of rat inferior vena cava. After implantation for 12 weeks, a thin continuous layer of endothelial cells and smooth muscle cells were lined with the vascular lumen and tunic media, respectively. Histology results showed that there were no signs of significant thrombogeneity and intima hyperplasia. This tissue engineered vascular substitute not only had enough tensile strength and good biocompatibility, but also advanced vascular regeneration. In the future, we suggest that this biodegradable vascular substitute will provide with the possibility in application on small diameter prosthetic grafts in artificial blood vessels. (c) 2006 Elsevier Ltd. All rights reserved.