Upconversion nanoparticles for sensitive and in-depth detection of Cu2+ ions
Upconversion nanoparticles for sensitive and in-depth detection of Cu2+ ions
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DOI：
10.1039/c2nr31570j
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发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Li, Chunxia;Liu, Jinliang;Zhang, Yong
Cu 2+离子的检测及其在生理过程中的亚细胞分布研究具有重要的意义，因为它们具有潜在的环境和生物应用价值。已经开发了一些基于荧光的传感器用于选择性检测Cu 2+离子，其基于特异性结合Cu 2+离子的有机荧光探针。然而，由于用于激发荧光探针的UV/可见光的短穿透深度，这些传感器不适合于在生物样品中进行检测。近红外（NIR）光的使用可以提供比可见光的穿透深度大一个数量级的穿透深度，然而，需要可以将NIR光转换为可见光的材料。基于荧光上转换原理，建立了一种简便的深度检测Cu ~（2+）的方法。将介孔二氧化硅壳包覆在上转换纳米颗粒（UCNPs）上，并将Cu 2+离子敏感的荧光探针罗丹明B酰肼掺入介孔二氧化硅中。在由NIR光激发时，UCNP发射可见光以激发Cu 2+敏感的荧光探针。由于UCNPs独特的光学性质及其将NIR光转换为可见光的能力，由于NIR光的低自发荧光和高穿透深度，这是一种用于复杂生物或环境样品中Cu 2+离子的灵敏和深入检测的可行方法。
Detection of Cu2+ ions and study of their subcellular distribution in physiological processes are of considerable significance because of their potential environmental and biological applications. Some fluorescence based sensors have been developed for selective detection of Cu2+ ions, based on organic fluorescent probes that specifically bind to Cu2+ ions. However, these sensors are not suitable for detection in biological samples due to the short penetration depth of UV/visible light used to excite the fluorescent probes. The use of near-infrared (NIR) light can afford penetration depths of an order of magnitude greater than that of visible light, however, a material that can convert NIR light to visible light is required. A facile method has been developed for in-depth detection of Cu2+ ions based on fluorescence upconversion. A mesoporous silica shell is coated on upconversion nanoparticles (UCNPs) and a Cu2+ ion sensitive fluorescent probe, rhodamine B hydrazide, is incorporated into the mesoporous silica. Upon excitation by a NIR light, the UCNPs emit visible light to excite the Cu2+-sensitive fluorescent probe. Because of the unique optical properties of UCNPs and their ability to convert NIR light to visible light, this is a feasible method for sensitive and in-depth detection of Cu2+ ions in a complex biological or environmental sample due to the low autofluorescence and the high penetration depth of NIR light.