A uterine fluid-mediated sperm-inhibitory system.
A uterine fluid-mediated sperm-inhibitory system.
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子宫液介导的精子抑制系统。
DOI：
10.1093/biolreprod/3.2.229
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发表时间：
1970
影响因子：
3.6
通讯作者：
S. Klebanoff
中科院分区：
文献类型：
--
作者：
D. Smith;S. Klebanoff
该报告表明，子宫液在某些条件下发挥精子抑制作用。此外，大鼠子宫液碘化物和过氧化氢洗涤精子悬浮在无钙离子的Krebs-Ringer磷酸果糖培养基pH 6.5导致在10分钟内完成的运动抑制。当精子单独在培养基中孵育，他们仍然完全能动2小时。删除过氧化氢从系统废除了精子抑制作用。当子宫液或碘被删除时，在长时间孵育时，运动的一些抑制是明显的。硫氰酸盐可替代碘离子，葡萄糖和葡萄糖氧化酶可替代过氧化氢，抑制率分别为94%和95%。子宫液可用LPO（乳过氧化物酶）制剂替代，或用MPO（髓过氧化物酶）替代，效果较差。这表明其精子抑制作用是由于其过氧化物酶含量。子宫液-碘化物-过氧化氢系统悬浮精子导致丙酮酸氧化减少。未洗涤的精子不受系统的影响，提示精浆中存在抑制剂。这一点得到了测试的证实，测试表明该系统受到过氧化氢酶以及叠氮还原型谷胱甘肽、半胱氨酸、麦角硫因和抗坏血酸等低分子量化合物的抑制。热稳定的低分子量抑制剂存在于精浆中，少量存在于子宫液中。
The report suggests that uterine fluid under certain conditions exerts a sperm inhibitory effect. The addition of rat uterine fluid iodide and hydrogen peroxide to washed spermatozoa suspended in a calcium ion-free Krebs-Ringer phosphate fructose medium pH 6.5 resulted in an inhibition of motility which was complete in 10 min. When the spermatozoa were incubated in medium alone they remained fully motile for 2 hr. Deletion of hydrogen peroxide from the system abolished the sperm-inhibitory effect. Some inhibition of motility was evident on prolonged incubation when either uterine fluid or iodide was deleted. Thiocyanate could substitute for iodide and glucose and glucose oxidase could substitute for hydrogen peroxide giving 94% and 95% inhibition respectively. The uterine fluid could be replaced by a preparation of LPO (lactoperoxidase) or less effectively by MPO (myeoperoxidase). This suggests that its sperm inhibitory effect is due to its perioxidase content. The uterine fluid-iodide-hydrogen peroxide system suspended spermatozoa resulted in a decrease in pyruvate oxidation. Unwashed spermatozoa were unaffected by the system suggesting inhibitors in seminal plasma. This was born out by tests which showed the system was inhibited by catalase and by such low-molecular-weight compounds as azide reduced glutathione cysterine ergothioneine and ascorbic acid. Heat-stable low-molecular-weight inhibitors are found in seminal plasma and to a lesser degree in uterine fluid.