Pterostilbene induces apoptosis through caspase activation in ovarian cancer cells
Pterostilbene induces apoptosis through caspase activation in ovarian cancer cells
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DOI：
10.12892/ejgo3024.2016
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发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Chen, Y.
中科院分区：
文献类型：
--
作者：
Dong, J.;Guo, H.;Chen, Y.
目的：白藜芦醇类似物白藜芦醇可提高生物利用度，其体外和体内研究表明具有抗氧化和抗癌作用。具有抗氧化性能的饮食化合物已被证明由于治疗应用而变得重要。此外，具有较高生物利用度的化合物在当前情况下表现出极大的兴趣。因此，本研究旨在探讨蕨类化合物对卵巢癌细胞株的细胞毒作用及其细胞死亡机制。材料和方法：通过细胞毒性试验、氧化应激水平、细胞内游离钙离子浓度、线粒体去极化、细胞周期分析和caspase3、8、9活性检测，观察蕨类化合物对SKOV-3细胞的作用。结果：研究表明，蕨类化合物在IC50-55微米范围内具有细胞毒作用。此外，刺五加还通过增强氧化应激、[Ca~(2+)]水平、线粒体去极化、亚G_1积聚以及caspase 3和9的激活来诱导细胞凋亡。结论：本研究首次证实了刺五加对卵巢癌细胞的细胞毒作用。
Aim: Pterostilbene, an analog of resveratrol increasing bioavailability has shown to offer antioxidant and anticancer properties in vitro and in vivo. Dietary compounds with anti-oxidant properties have been shown to gain importance due to therapeutic applications. In addition, compounds with higher bioavailability levels show great interest in present scenario. Thus, the present study aimed at investigating the cytotoxic role of pterostilbene and its mechanism of cell death in ovarian cancer cells line. Materials and Methods: The effect of pterostilbene was determined on SKOV-3 cells, by cytotoxicity assays, oxidative stress levels, [Ca2+] levels, mitochondrial depolarization, cell cycle analysis and caspase 3, 8, and 9 activities. Results: The study revealed that pterostilbene offered cytotoxic effect at a concentration of IC50-55 uM. Further, pterostilbene induced reactive oxygen species (ROS) mediated intrinsic pathway of apoptosis through enhancing oxidative stress, [Ca2+] levels, mitochondrial depolarization, Sub G1 accumulation, and activation of caspase 3 and 9. Conclusion: The study demonstrates for the first time the cytotoxic potential of pterostilbene against ovarian cancer cells.