Identification of vaccine candidates from differentially expressed outer membrane proteins of Vibrio alginolyticus in response to NaCl and iron limitation.
Identification of vaccine candidates from differentially expressed outer membrane proteins of Vibrio alginolyticus in response to NaCl and iron limitation.
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DOI：
10.1016/j.fsi.2010.07.027
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发表时间：
2010-11
影响因子：
4.7
通讯作者：
Xiao-peng Xiong;Bing Zhang;Man-jun Yang;Ming-Zhi Ye;Xuan-xian Peng;Hui Li
中科院分区：
文献类型：
--
作者：
Xiao-peng Xiong;Bing Zhang;Man-jun Yang;Ming-Zhi Ye;Xuan-xian Peng;Hui Li
溶藻弧菌是造成水产养殖业巨大损失和人类临床传播病例的病原。由于疫苗是有效预防细菌感染的有效途径，因此鉴定高效的候选疫苗以控制藻毒弧菌感染一直受到高度关注。本研究首先利用免疫印迹法研究了溶藻弧菌（V. alginolyticus）外膜蛋白（OM蛋白）在NaCl浓度和铁限制下的变化，然后在免疫后通过细菌攻击鉴定了这些改变的OM蛋白的保护活性。10个OM蛋白对渗透压变化或铁限制有差异表达，其中VA2212、OmpV、VPA1186、OmpU、VPA1644、VA1061、VA1631和VPA0860对渗透压的响应明显改变，VPA1186、OmpU、OmpV、VA0449、VPA0860、VPA1435和VA1631对铁限制有响应。在10个OM蛋白中，VA1061、OmpU、VPA1435和VPA0860可作为抗溶藻弧菌体内感染的有效候选疫苗。进一步的结果表明，VA1061和VPA0860是优势抗原，在活的或灭活的全细胞疫苗中可以刺激宿主产生比其他两种更强的抗体应答。这些结果不仅扩展了对渗透压、铁反应蛋白的认识，而且为鉴定适合用于疫苗开发的保护蛋白提供了有价值的策略。
Vibrio alginolyticus is the etiological agent that causes great losses in aquacultures and clinical emanating cases in humans. Identification of highly efficient vaccine candidates to control V. alginolyticus infection has been highly concerned since vaccines offer a powerful approach to provide efficient protection from bacterial infections. In the present study, we firstly investigated the altered outer membrane proteins (OM proteins) of V. alginolyticus in response to NaCl concentrations and iron limitation using Western blotting, and then identified the protective activity of these altered OM proteins by bacterial challenge post immunization. Ten OM proteins were differentially expressed in response to the osmolarity changing or/and iron limitation, in which VA2212, OmpV, VPA1186, OmpU, VPA1644, VA1061, VA1631 and VPA0860 were markedly altered in response to osmolarity, and VPA1186, OmpU, OmpV, VA0449, VPA0860, VPA1435 and VA1631 were determined to be iron-limited responsive proteins. Out of the ten OM proteins, VA1061, OmpU, VPA1435 and VPA0860 could be effective vaccine candidates against infection by V. alginolyticus in vivo. Further results indicated that VA1061 and VPA0860 were dominant antigens and could stimulate hosts to produce stronger antibody response than other two in live or inactivated whole-cell vaccines. These results not only expand knowledge on osmolarity-, iron-responsive proteins, but also provide a valuable strategy for identify protective proteins suitable for use in vaccine development.