Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone
Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone
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DOI：
10.1016/j.vaccine.2015.06.112
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发表时间：
2015-08-20
期刊：
影响因子：
5.5
通讯作者：
Moste, Catherine
中科院分区：
文献类型：
--
作者：
Medina, Julie;Boukhebza, Houda;Moste, Catherine
流感抗原产生的产量在疫苗株之间可能显著不同;例如，在2009年流感大流行期间制备的A/加州/07/09（H1N1）-X179 A疫苗病毒与其他季节性HI NI抑制剂相比在鸡蛋中呈现低抗原产量。在这项研究中，使用Vero/CHOK 1细胞系的混合物通过反向遗传学拯救了表达HA和NA基因的双嵌合病毒，该双嵌合病毒具有A/波多黎各/8/34（H1 N1）（PR 8）和X179 A结构域（Medina等人[7]）。通过单向免疫扩散（SRID）（定量流感疫苗中HA蛋白的参考方法）测定，证明获得的双嵌合病毒在鸡蛋中产生的HA量远大于X179 A。这种使用PR 8骨架衍生的嵌合糖蛋白的优化病毒可用作疫苗生产的改良种子病毒。(C)2015爱思唯尔有限公司版权所有。
The yield of influenza antigen production may significantly vary between vaccine strains; for example the A/California/07/09 (H1N1)-X179A vaccine virus, prepared during 2009 influenza pandemic, presented a low antigen yield in eggs compared to other seasonal HI NI reassortants. In this study a bi-chimeric virus expressing HA and NA genes with A/Puerto Rico/8/34 (H1N1) (PR8) and X179A domains was rescued by reverse genetics using a mixture of Vero/CHOK1 cell lines (Medina et al. [7]). The bi-chimeric virus obtained demonstrated to yield much larger amounts of HA than X179A in eggs as measured by single-radial-immunodiffusion (SRID), the reference method to quantify HA protein in influenza vaccine. Such kind of optimized virus using PR8 backbone derived chimeric glycoproteins could be used as improved seed viruses for vaccine production. (C) 2015 Elsevier Ltd. All rights reserved.