Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells.
Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells.
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DOI：
10.1111/cas.12746
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发表时间：
2015-10
期刊：
影响因子：
5.7
通讯作者：
Nakagawara A
中科院分区：
文献类型：
--
作者：
Islam SM;Suenaga Y;Takatori A;Ueda Y;Kaneko Y;Kawana H;Itami M;Ohira M;Yokoi S;Nakagawara A
Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-IN cells formed induced pluripotent stem cell-like colonies (SH-IN 4F colonies) at approximately 6 weeks following transduction. These SH-IN 4F colonies were alkaline phosphatase-positive. Array comparative genomic hybridization analysis indicated identical genomic aberrations in the SH-IN 4F cells as in the parental cells. SH-IN 4F cells had the ability to differentiate into the three embryonic germ layers in vitro, but rather formed NBs in vivo. Furthermore, SH-IN 4F cells exhibited resistance to cisplatin treatment and differentiated into endothelial-like cells expressing CD31 in the presence of vascular endothelial growth factor. These results suggest that SH-IN 4F cells are partially reprogrammed NB cells, and could be a suitable model for investigating the plasticity of aggressive tumors.
