Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants
Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants
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DOI：
10.1046/j.1365-313x.2000.00787.x
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发表时间：
2000-08-01
期刊：
影响因子：
7.2
通讯作者：
Izui, K
中科院分区：
文献类型：
--
作者：
Saijo, Y;Hata, S;Izui, K
水稻钙依赖蛋白激酶基因OsCDPK 7在低温和盐胁迫下被诱导表达。为了阐明OsCDPK 7的生理功能，我们产生了具有改变的蛋白质水平的转基因水稻植株。这些植物对低温和盐/干旱胁迫的耐受程度与OsCDPK 7的表达水平密切相关。因此，OsCDPK 7被证明是一个积极的调节剂，共同参与水稻对这两种胁迫的耐受性。OsCDPK 7的过表达增强了一些应激反应基因对盐/干旱的诱导，但对寒冷没有影响。因此，这表明导致耐寒性和耐盐/干旱性的下游途径彼此不同。似乎至少有两种不同的途径通常使用单一的CDPK，通过未知的翻译后调节机制维持信号传导特异性。这些结果表明，CDPK活性的简单操纵在植物改良方面具有巨大的潜力。
A rice gene encoding a calcium-dependent protein kinase (CDPK), OsCDPK7, was induced by cold and salt stresses. To elucidate the physiological function of OsCDPK7, we generated transgenic rice plants with altered levels of the protein. The extent of tolerance to cold and salt/drought stresses of these plants correlated well with the level of OsCDPK7 expression. Therefore, OsCDPK7 was shown to be a positive regulator commonly involved in the tolerance to both stresses in rice. Over-expression of OsCDPK7 enhanced induction of some stress-responsive genes in response to salinity/drought, but not to cold. Thus, it was suggested that the downstream pathways leading to the cold and salt/drought tolerance are different from each other. It seems likely that at least two distinct pathways commonly use a single CDPK, maintaining the signalling specificity through unknown post-translational regulation mechanisms. These results demonstrate that simple manipulation of CDPK activity has great potential with regard to plant improvement.