Cell Cycle Arrest by Hybrid Liposomes for Human Lung Carcinoma Cells
Cell Cycle Arrest by Hybrid Liposomes for Human Lung Carcinoma Cells
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DOI：
10.4172/2157-2518.1000157
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发表时间：
2014-01
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影响因子：
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通讯作者：
Y. Komizu;Mamiko Yukihara;Y. Matsumoto;R. Ueoka
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文献类型：
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作者：
Y. Komizu;Mamiko Yukihara;Y. Matsumoto;R. Ueoka
采用超声法制备了L-α-二肉豆蔻酰磷脂酰胆碱与聚氧乙烯（23）十二烷基醚的复合脂质体（HL）。本研究探讨HL对人非小细胞肺癌细胞周期和凋亡的影响。HL可诱导人非小细胞肺癌细胞（A549、H460和H23）发生G 0/G1期阻滞和凋亡。HL处理还导致细胞周期蛋白依赖性激酶抑制剂p21 WAF 1/CIP 1和p27 KIP 1的诱导以及细胞周期蛋白D1和E的蛋白表达降低。值得注意的是，用HL处理A549细胞以时间和剂量依赖性方式抑制Akt的磷酸化。HL处理抑制A549细胞丝状伪足的形成。这些结果表明HL诱导的细胞周期阻滞在G 0/G1期可能与cdk抑制剂p21和p27通过阻断Akt信号传导而上调有关
Hybrid liposomes (HL), composed of L-α-dimyristoylphosphatidylcholine and polyoxyethylene(23) dodecyl ethers, were simply prepared by the sonication method. In this study, we investigated the effects of HL on cell cycle and apoptosis in human non-small cell lung cancer cells. Induction of cell cycle arrest at the G0/G1 phase and apoptosis by HL were observed in human non-small cell lung cancer cells (A549, H460 and H23). HL treatment also resulted in the induction of cyclin-dependent kinases inhibitor p21WAF1/CIP1 and p27KIP1 and a decrease in the protein expressions of cyclins D1 and E. It is noteworthy that the treatment of A549 cells with HL inhibited phosphorylation of Akt in a time- and dose-dependent manner. Furthermore, HL treatment inhibited the filopodia formation in A549 cells. These results suggest that HL-induced cell cycle arrest at the G0/G1 phase could be associated by the up-regulation of cdk inhibitor p21 and p27 through blocking Akt signaling